Sensitive Phosphorylation ELISA Kits

Cambridge Bioscience offers a range of phosphorylation ELISA kits for many different targets to enable the fast and sensitive monitoring of activation or function of important biological pathways in cell lysate samples.

Benefits of Using the Phosphorylation ELISA Kits
• High sensitivity
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient & non-radioactive material
• Simultaneously measure phosphorylated protein & pan protein
• High quality
• Cost-effective
• Complete kit with pre-coated 96 well plate

Phosphorylation ELISA Kit Workflow

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phosphorylated protein. An specific capture antibody (anti-pan-antibody or anti-phosphorylated antibody) has been coated onto a 96-well plate. Samples are pipetted into the wells and target protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phosphorylated antibody or anti-pan-antibody is used to detect target protein. After washing away unbound antibody, secondary antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of target bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Sensitive Phosphorylation ELISA Kits Citation List