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Robust, small RNA quantification - QuantiMir™

The sensitive and robust QuantiMir™ kit enables the generation of PCR-ready cDNA from just 10 pg of total RNA. With a simple protocol, the QuantiMir™ kit can be used for the profiling of any small RNA and is more sensitive, quantitative and easier to perform than Northern or RNase protection experiments.

Benefits of using the QuantiMir™ kit
• Three step procedure
• Two hour protocol
• Highly sensitive
• All small RNAs rapidly tagged and converted into detectable cDNA for qPCR
• Enough RNA to cDNA converted for less than 5,000 qPCR measurements
• High-throughput screening compatible
• Three reference assays included

How QuantiMir™ works
QuantiMir™ enables highly efficient poly(A) tailing and reverse transcription in a single reaction tube producing the uniform cDNA synthesis of microRNAs. Optimised reaction conditions and buffer components maximise cDNA yield enabling the use of between micrograms and picograms of input total RNA. The universal 3' tag sequence incorporated during reverse transcription enables the ease of scalable and accurate microRNA expression analysis by qPCR.

Quantimir Process
QuantiMir™ applications
cDNA produced by QuantiMir™ can be used for many applications including:
• Profiling microRNA expression changes in cancer
• Detecting microRNAs involved in cellular differentiation
• Genome-wide qPCR microRNA expression profiling

Associated panels
We offer a range of associated panels for use with the QuantiMir™ kits including
• OncoMir microRNA qPCR array - apoptosis, differentiation and cancer (find out more)
• Genome wide microRNA qPCR array panels - human, mouse and rat
• Stem cell microRNA qPCR array - stem cell self-renewal, haemopoiesis, neural development and tissue patterning

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
QuantiMir Kit RA420A-1 · System Biosciences
RA420A-1
System Biosciences
20 reactions £535.00
20 reactions
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Cells-to-Cts + QuantiMir complete Kit RA422A-1 · System Biosciences
RA422A-1
System Biosciences
20 reactions £565.00
20 reactions
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QuantiMir 3'-Universal Primer (10,000 qPCR reactions) RA420AU-3 · System Biosciences
RA420AU-3
System Biosciences
10000 assays £115.00
10000 assays
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RA420A-hU6 QuantiMir Human U6--50 assays RA420A-hU6 · System Biosciences
RA420A-hU6
System Biosciences
50 assays £91.00
50 assays
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RA420A-mU6 QuantiMir Mouse U6--50 assays RA420A-mU6 · System Biosciences
RA420A-mU6
System Biosciences
50 assays £91.00
50 assays
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RA420A-RNU1 QuantiMir RNU1 (U1)--50 assays RA420A-RNU1 · System Biosciences
RA420A-RNU1
System Biosciences
50 assays £91.00
50 assays
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RA420A-RNU43 QuantiMir RNU43--50 assays RA420A-RNU43 · System Biosciences
RA420A-RNU43
System Biosciences
50 assays £91.00
50 assays
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RA420A-miR-16 QuantiMir miR-16 --50 assays RA420A-miR-16 · System Biosciences
RA420A-miR-16
System Biosciences
50 assays £91.00
50 assays
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RA420A-rU6 QuantiMir Rat U6--50 assays RA420A-rU6 · System Biosciences
RA420A-rU6
System Biosciences
50 assays £91.00
50 assays
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