Robust, small RNA quantification - QuantiMir™

The sensitive and robust QuantiMir™ kit enables the generation of PCR-ready cDNA from just 10 pg of total RNA. With a simple protocol, the QuantiMir™ kit can be used for the profiling of any small RNA and is more sensitive, quantitative and easier to perform than Northern or RNase protection experiments.

Benefits of using the QuantiMir™ kit
• Three step procedure
• Two hour protocol
• Highly sensitive
• All small RNAs rapidly tagged and converted into detectable cDNA for qPCR
• Enough RNA to cDNA converted for less than 5,000 qPCR measurements
• High-throughput screening compatible
• Three reference assays included

How QuantiMir™ works
QuantiMir™ enables highly efficient poly(A) tailing and reverse transcription in a single reaction tube producing the uniform cDNA synthesis of microRNAs. Optimised reaction conditions and buffer components maximise cDNA yield enabling the use of between micrograms and picograms of input total RNA. The universal 3' tag sequence incorporated during reverse transcription enables the ease of scalable and accurate microRNA expression analysis by qPCR.


QuantiMir™ applications
cDNA produced by QuantiMir™ can be used for many applications including:
• Profiling microRNA expression changes in cancer
• Detecting microRNAs involved in cellular differentiation
• Genome-wide qPCR microRNA expression profiling

Associated panels
We offer a range of associated panels for use with the QuantiMir™ kits including
• OncoMir microRNA qPCR array - apoptosis, differentiation and cancer (find out more)
• Genome wide microRNA qPCR array panels - human, mouse and rat
• Stem cell microRNA qPCR array - stem cell self-renewal, haemopoiesis, neural development and tissue patterning

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