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Rapid & efficient Cold Fusion DNA Cloning

This range of simple and highly efficient kits from System Biosciences enable the rapid and direct cloning of any PCR product(s) to any linearised expression vector, at any site. By joining single and multiple PCR fragment(s) into a linearised destination vector in a single step, these kits enable the direct cloning of any PCR product without the use of restriction enzymes and ligase.

Benefits of using the Cold Fusion Cloning kits
• Clone any insert at any site into any vector
• Highly efficient - greater than 95 percent positive cloning rates
• Convenient - one tube reaction
• Fast - 20 minute procedure
• Robust
• Highly accurate
• No restriction enzyme digest required
• No ligation
• Compatible with high throughput cloning applications
• Capable of cloning single fragments and also multiple fragments in the order you choose

Testimonials
“I have designed and made hundreds and hundreds of clones using the traditional and not so traditional methods since I started doing research. I must say the Cold Fusion kit makes it way too easy to design a vector or new construct – it’s a really good product”
Dr. Hidevaldo B. Machado, UCLA

Cold Fusion Cloning workflow


The 5x Cold Fusion Master mix contains a proprietary blend of enzymes that prepare the ends of DNA fragments for sequence-directed alignment. Compatible DNA ends are then efficiently fused and produce vector clones with great accuracy.

Material available for download
Cold Fusion Cloning kit manual
Cold Fusion Quick protocol
Cold Fusion Cloning kit product sheet

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Cold Fusion Cloning Kit with Competent Cells (10 rxns) International Sales Only MC010A-1 · System Biosciences
MC010A-1
System Biosciences
10 reactions £229.00
10 reactions
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Cold Fusion Cloning Kit with Competent Cells (20 rxns) International Sales Only MC100A-1 · System Biosciences
MC100A-1
System Biosciences
20 reactions £418.00
20 reactions
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Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only MC101A-1 · System Biosciences
MC101A-1
System Biosciences
50 reactions £961.00
50 reactions
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Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only MC096A-1 · System Biosciences
MC096A-1
System Biosciences
10 reactions £1690.00
10 reactions
view