The LiveLight™ range of photostable cell culture media from Cell Guidance Systems enables prolonged live cell imaging without the loss of cell viability. With phototoxic components found in traditional media removed or replaced, LiveLight has been specifically designed to replace standard culture media during prolonged light exposure applications including FACS and fluorescence microscopy.
Benefits of using LiveLight media
• Reduces photo-bleaching
• Reduces auto-fluorescence
• Increases cell viability
LiveLight media range
LiveLight cell culture system encompasses three different photostable products:
• MEMO® medium replaces DMEM during light exposure
• NEUMO® medium replaces Neurobasal® media during light exposure
• SOS® supplement replaces B-27® and similar neuronal supplements (e.g. N2, SATO, NS21) during light exposure
OPC, cortical neuron and hippocampan neuron growth with LiveLight
Shining light (470 nm) onto OPCs for optogenetics in standard cell culture media kills almost all cells (A and B) . Using MEMO® and SOS® completely prevents OPC death from optogenetic blue light. Viability is also increased in (C) cortical and (D) hippocampal neurons.
LiveLight comparison to Neurobasal® and B-27®
Successful FACS sorting, culturing and differentiation of viable GFP and RFP labelled central nervous system stem cells (OPCs) from adult mouse brain is only achievable with NEUMO® and SOS® compared to Neurobasal® and B-27®. Data courtesy of Abbe Crawford and Prof. Robin Franklin of the Wellcome Trust-MRC Cambridge Stem Cell Institute. Bar = 100 mm.
Important information
• MEMO should be used only prior to and during exposure to light. At other times, standard DMEM should be used. If B-27® or similar neuronal supplements are used in combination with DMEM, these should be replaced with SOS.
• NEUMO should be used only prior to and during exposure to light. At other times, Neurobasal® medium should be used. Please see user guide for instructions.
Material available for download
MEMO Photostable Cell Culture Medium user guide
NEUMO Photostable Cell Culture Medium user guide
SOS Cell Culture Supplement user guide
LiveLight citations
Neurobasal and B-27 are registered trademarks of Life Technologies Corp.
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| MEMO M06-100 · Cell Guidance Systems | M06-100 Cell Guidance Systems |
100 ml |
£56.00
100 ml
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| MEMO M06-500 · Cell Guidance Systems | M06-500 Cell Guidance Systems |
500 ml |
£112.00
500 ml
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|
| NEUMO M07-100 · Cell Guidance Systems | M07-100 Cell Guidance Systems |
100 ml |
£56.00
100 ml
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|
| NEUMO M07-500 · Cell Guidance Systems | M07-500 Cell Guidance Systems |
500 ml |
£112.00
500 ml
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|
| SOS M09-50 · Cell Guidance Systems | M09-50 Cell Guidance Systems |
50 ml |
£200.00
50 ml
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|
