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Photostable cell culture media: LiveLight

The LiveLight™ range of photostable cell culture media from Cell Guidance Systems enables prolonged live cell imaging without the loss of cell viability. With phototoxic components found in traditional media removed or replaced, LiveLight has been specifically designed to replace standard culture media during prolonged light exposure applications including FACS and fluorescence microscopy. 

Benefits of using LiveLight media
• Reduces photo-bleaching 
• Reduces auto-fluorescence
• Increases cell viability

LiveLight media range
LiveLight cell culture system encompasses three different photostable products:
• MEMO® medium replaces DMEM during light exposure
• NEUMO® medium replaces Neurobasal® media during light exposure
• SOS® supplement replaces B-27® and similar neuronal supplements (e.g. N2, SATO, NS21) during light exposure

OPC, cortical neuron and hippocampan neuron growth with LiveLight

 OPC, cortical neuron and hippocampan neuron growth with LiveLight

Shining light (470 nm) onto OPCs for optogenetics in standard cell culture media kills almost all cells (A and B) . Using MEMO® and SOS® completely prevents OPC death from optogenetic blue light. Viability is also increased in (C) cortical and (D) hippocampal neurons.

LiveLight comparison to Neurobasal® and B-27®

 LiveLight comparison to Neurobasal® and B-27®


Successful FACS sorting, culturing and differentiation of viable GFP and RFP labelled central nervous system stem cells (OPCs) from adult mouse brain is only achievable with NEUMO® and SOS® compared to Neurobasal® and B-27®. Data courtesy of Abbe Crawford and Prof. Robin Franklin of the Wellcome Trust-MRC Cambridge Stem Cell Institute. Bar = 100 mm.

Important information
• MEMO should be used only prior to and during exposure to light. At other times, standard DMEM should be used. If B-27® or similar neuronal supplements are used in combination with DMEM, these should be replaced with SOS.
• NEUMO should be used only prior to and during exposure to light. At other times, Neurobasal® medium should be used. Please see user guide for instructions.

Material available for download
MEMO Photostable Cell Culture Medium user guide
NEUMO Photostable Cell Culture Medium user guide
SOS Cell Culture Supplement user guide

LiveLight citations

Neurobasal and B-27 are registered trademarks of Life Technologies Corp. 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
MEMO M06-100 · Cell Guidance Systems
M06-100
Cell Guidance Systems
100 ml £56.00
100 ml
view
MEMO M06-500 · Cell Guidance Systems
M06-500
Cell Guidance Systems
500 ml £112.00
500 ml
view
NEUMO M07-100 · Cell Guidance Systems
M07-100
Cell Guidance Systems
100 ml £56.00
100 ml
view
NEUMO M07-500 · Cell Guidance Systems
M07-500
Cell Guidance Systems
500 ml £112.00
500 ml
view
SOS M09-50 · Cell Guidance Systems
M09-50
Cell Guidance Systems
50 ml £200.00
50 ml
view