CBL-BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for two hours at 37ºC. The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody. The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.
Benefits of using OxiSelect™ Protein Carbamylation Sandwich ELISA Kit
• Rapid detection and quantitation of protein carbamylation
• 1.5 ng/mL sensitivity detection limit of CBL-BSA
Material available for download
OxiSelect™ Protein Carbamylation Sandwich ELISA Kit manual
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|OxiSelect™ Protein Carbamylation Sandwich ELISA Kit STA-877 · Cell Biolabs||