mRNA reprogramming factors

The mRNAExpress™ mRNA synthesis kit is a non-integrative, high efficiency mRNA-based approach for generating iPSCs. 

Benefits of Using mRNAExpress™ Stem Cell Factors
• Single cocktail containing all the factors for reprogramming
• High efficiency transfection with RNAFection™
• Low toxicity transfection with RNAFection™
• Eliminates mutagenesis risk - non-integrative technology
• Titratable, reliable & reproducible iPSC reprogramming
• Rapid & transient protein expression levels

Technology
Uses Oct-4, Sox-2, Klf-4, Lin-28 & Nanog mRNA constructs to reprogram somatic cells to a pluripotent state. The mRNA constructs have enhanced stability through the incorporation of modified nucleobases & increased translatability with 5' cap analogs & 3' polyA tails. Transfection using RNAFection™ solution ensures high efficiency & low toxicity across a broad range of cell lines, including fibroblasts & lymphocytes. Protein expression from the mRNAs is seen within 5 hours of transfection & peaks from 24 - 48 hours.

Example Data
HEK293 cells were transfected with 2 µg of the mRNAExpress™ transcript indicated using the RNAFection™ reagent. Autofluorescence (GFP) & immunostaining of the factors are shown in green & blue is DAPI staining for nuclei. The mRNAExpress™ transcripts are high quality RNAs that show expected banding with the appropriate sizes (see lower panel).

mRNAExpress

Material Available for Download
mRNAExpress™ mRNA Synthesis Kit Datsheet
mRNAExpress™ mRNA Synthesis Kit User Manual
mRNAExpress and RNAFection Protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
pMRNAxp mRNAExpress Vector MR000PA-1 · System Biosciences
MR000PA-1
System Biosciences
10 µg £757.00
10 µg
view
mRNAExpress mRNA Synthesis kit (5 reactions) MR-KIT-1 · System Biosciences
MR-KIT-1
System Biosciences
5 reactions £982.00
5 reactions
view
RNAfection, RNA transfection reagent 50 ul MR750A-1 · System Biosciences
MR750A-1
System Biosciences
50 µl £144.00
50 µl
view
mRNAExpress hOct4 transcript 10 ug MR100A-1 · System Biosciences
MR100A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress hOct4 transcript 20 ug MR100A-2 · System Biosciences
MR100A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExmRNAExpress hSox2 transcript 10 ug MR200A-1 · System Biosciences
MR200A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExmRNAExpress hSox2 transcript 20 ug MR200A-2 · System Biosciences
MR200A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress hKlf4 transcript 10 ug MR300A-1 · System Biosciences
MR300A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress hKlf4 transcript 20 ug MR300A-2 · System Biosciences
MR300A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress hcMyc transcript 10 ug MR400A-1 · System Biosciences
MR400A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress hcMyc transcript 20 ug MR400A-2 · System Biosciences
MR400A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress hLin28 transcript 10 ug MR500A-1 · System Biosciences
MR500A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress hLin28 transcript 20 ug MR500A-2 · System Biosciences
MR500A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress hNanog transcript 10 ug MR600A-1 · System Biosciences
MR600A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress hNanog transcript 20 ug MR600A-2 · System Biosciences
MR600A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress GFP transcript 10 ug MR700A-1 · System Biosciences
MR700A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress GFP transcript 20 ug MR700A-2 · System Biosciences
MR700A-2
System Biosciences
20 µg £297.00
20 µg
view
mNAExpress RFP transcript 10 ug MR800A-1 · System Biosciences
MR800A-1
System Biosciences
10 µg £207.00
10 µg
view
mRNAExpress RFP transcript 20 ug MR800A-2 · System Biosciences
MR800A-2
System Biosciences
20 µg £297.00
20 µg
view
mNAExpress hMyoD transcript 10 ug MR900A-1 · System Biosciences
MR900A-1
System Biosciences
10 µg £207.00
10 µg
view
mNAExpress hMyoD transcript 10 ug MR900A-2 · System Biosciences
MR900A-2
System Biosciences
20 µg £297.00
20 µg
view
mRNAExpress Tail PCR mix (5uM) MR-TAIL-PR · System Biosciences
MR-TAIL-PR
System Biosciences
100 µl £102.00
100 µl
view