This kit can be used to measure mitochondrial reactive oxygen species (ROS) directly in living adherent or suspension cells. Targeted primarily to the mitochondrion, the mitochondrial ROS assay kit is better suited to single out ROS from other sources in the cell compared to less location-specific DHE and DCFDA dyes.
Benefits of using this Mitochondrial ROS Detection Assay Kit
• Measure mitochondrial ROS directly in living cells
• Includes antimyin A as a positive control
• Flow cytometry, fluorescence and plate-based methods
H9C2 cells were stained with 0.62 µM mitochondrial ROS detection reagent following the adherent cells protocol treated with 3 µM antimycin A (A) or vehicle (B). Images were captured one hour after treatment with antimycin A using Biotek® Cytation™ 5 Multi-Mode Reader.
THP-1 cells were stained with 0.5 µM mitochondrial ROS detection reagent following the suspension cells protocol. Cells were treated with 1 µM antimycin A or vehicle and incubated for one hour before measuring on a flow cytometer.
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