www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

miR-ID® microRNA detection platform

The miR-ID® assays from SomaGenics enable sensitive and highly specific quantification of low abundance small RNAs, such as microRNAs, from total RNA isolates. This novel platform uses a circularisation-based real time quantitative PCR (qPCR) method for quantifying microRNAs (miRNAs) and related isomiRs and isoforms. This technology is compatible with all sample sources, including total RNA, cell lysates and tissue lysates.

Benefits of using the miR-ID® microRNA detection platform
• Ideal for absolute or relative miRNA quantification
• Distinguish 2’Omethyl modifications at the 3’-terminus of miRNAs
• Compatible with all sample sources including total RNA, cell lysates and tissue lysates

miR-ID® technology

mir-ID schematic

Step 1: Circularisation of a linear miRNA; Step 2: Reverse transcription mediated by rolling circle amplification to obtain multimeric cDNA; Step3: Quantification of the amplicon by RT-qPCR using highly sequence-specific 5’-overlapping primers and SYBR Green detection.

miR-Direct® workflow

mir-Direct timeline

All steps up to quantitative PCR are performed in multiplex in a single tube. Note that this schematic includes the miR-ID® steps.

miR-ID® is highly sensitive and specific

miR-ID® is highly sensitive and specific

For example, the closely related miRNAs 34a, b, and c are well discriminated by miR-ID®. To determine the relative Ct values listed in the table, the difference in Ct values for the intended target and their related isoforms was converted into a linear percentage, with the fully matched reaction normalised to 100%. All non-specific detection is well below 1% for these assays. The dilution curves indicate that all three assays have a dynamic detection range of 8 logs.

miR-ID® detects end-modified small RNAs

miR-ID® detects end-modified small RNAs

miR-ID® quantitatively discriminates between small RNAs with an identical sequence but differing terminal modifications. This is particularly useful in plant miRNAs, where discrimination between 2-O-methyl modified and unmodified miRNA is of considerable interest.

miR-ID® can quantitatively detect 2' O-methyl modifications at the 3’-terminus of miRNAs. By selecting enzymes that circularize either both types of miRNAs (with 2’-OMe or 2’-OH forms on the 3’termini), shown in green, or 2’-OH forms only (shown in red), these two end-modifications can be distinguished. In this example, synthetic let-7b miRNAs with the two respective 3’ termini were assayed and quantification with the enzyme circularizing both forms were set to 100%

Material available for download
miR-ID® miRNA quantification assays protocol
miR-ID® brochure
miR-ID®: a sensitive and specific method for microRNA quantification
Paper: miR-ID: A novel, circularization-based platform for detection of microRNAs

New miR-ID® assays are continually being added. If you do not see your miRNA on our list or are interested in detecting novel isoforms, please contact us as it may be available shortly or by special order.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
miR-ID-ath-miR159 100-00011 · SomaGenics
100-00011
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-cel-lin-4 100-00010 · SomaGenics
100-00010
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-cel-miR-39 100-00012 · SomaGenics
100-00012
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-let-7a-5p 100-00015 · SomaGenics
100-00015
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-let-7d-5p 100-00016 · SomaGenics
100-00016
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-let-7f-5p 100-00086 · SomaGenics
100-00086
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-let-7g-5p 100-00017 · SomaGenics
100-00017
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-let-7i-5p 100-00087 · SomaGenics
100-00087
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-100-5p 100-00089 · SomaGenics
100-00089
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-103a-3p 100-00061 · SomaGenics
100-00061
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-106a-5p 100-00018 · SomaGenics
100-00018
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-106b-5p 100-00073 · SomaGenics
100-00073
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-107 100-00074 · SomaGenics
100-00074
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-10a-5p 100-00019 · SomaGenics
100-00019
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-10b-5p 100-00020 · SomaGenics
100-00020
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-122-5p 100-00021 · SomaGenics
100-00021
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-125a-5p 100-00022 · SomaGenics
100-00022
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-125b-5p 100-00023 · SomaGenics
100-00023
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-126-3p 100-00024 · SomaGenics
100-00024
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-126-5p 100-00025 · SomaGenics
100-00025
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-127-3p 100-00069 · SomaGenics
100-00069
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-128-3p 100-00026 · SomaGenics
100-00026
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-141-3p 100-00027 · SomaGenics
100-00027
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-142-3p 100-00028 · SomaGenics
100-00028
SomaGenics
100 reactions £203.00
100 reactions
view
miR-ID-hsa-miR-143-3p 100-00029 · SomaGenics
100-00029
SomaGenics
100 reactions £203.00
100 reactions
view