MemBrite™ Fix Cell Surface Staining Kits

MemBrite™ Fix Cell Surface Staining Kits from Biotium use new covalent cell surface stains, which react rapidly and irreversibly with cell surface proteins. The stains can withstand formaldehyde or alcohol fixation and detergent permeabilisation.

Benefits of using the MemBrite™ Fix Cell Surface Staining Kits
• Quick procedure
• Uniform live/ fixed cell surface staining
• Covalent labelling can be fixed and permeabilised for IF staining
• Stain yeast and Gram-positive bacteria
• Choose from eight bright and photostable dye colours from blue to near-IR
• Trial sizes available

MemBrite Procedure

MemBrite™ Fix has excellent tolerance for methanol fixation

MemBrite™ Fix has excellent tolerance for methanol fixation

Cells were labelled with the indicated dye for five minutes at 37°C, then fixed with ice-cold methanol for five minutes at -20°C.

MemBrite™ Fix labels the cell surface rapidly and specifically, and tolerates fixation and permeabilisation

MemBrite™ Fix labels the cell surface rapidly and specifically, and tolerates fixation and permeabilisation

HeLa cells were labelled with MemBrite™ Fix 640/660 for five minutes at 37°C or 30 minutes at 4°C. Cells were then fixed with 4% paraformaldehyde, followed by permeabilisation with Triton X-100, or fixed with ice cold methanol. Cells labelled at 4°C were also fixed and permeabilised at 4°C.

Material available for download
Membrane and Cell Surface Stains Brochure

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
MemBrite™ Fix 405/430 Cell Surface Staining Kit, 500 Reactions BT30092 · Biotium
BT30092
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 405/430 Cell Surface Staining Kit, 100 Reactions BT30092-T · Biotium
BT30092-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 488/515 Cell Surface Staining Kit, 500 Reactions BT30093 · Biotium
BT30093
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 488/515 Cell Surface Staining Kit, 100 Reactions BT30093-T · Biotium
BT30093-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 543/560 Cell Surface Staining Kit, 500 Reactions BT30094 · Biotium
BT30094
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 543/560 Cell Surface Staining Kit, 100 Reactions BT30094-T · Biotium
BT30094-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 568/580 Cell Surface Staining Kit, 500 Reactions BT30095 · Biotium
BT30095
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 568/580 Cell Surface Staining Kit, 100 Reactions BT30095-T · Biotium
BT30095-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 594/615 Cell Surface Staining Kit, 500 Reactions BT30096 · Biotium
BT30096
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions BT30096-T · Biotium
BT30096-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 640/660 Cell Surface Staining Kit, 500 Reactions BT30097 · Biotium
BT30097
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 640/660 Cell Surface Staining Kit, 100 Reactions BT30097-T · Biotium
BT30097-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 660/680 Cell Surface Staining Kit, 500 Reactions BT30098 · Biotium
BT30098
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 660/680 Cell Surface Staining Kit, 100 Reactions BT30098-T · Biotium
BT30098-T
Biotium
1 kit £176.00
1 kit
view
MemBrite™ Fix 680/700 Cell Surface Staining Kit, 500 Reactions BT30099 · Biotium
BT30099
Biotium
1 kit £605.00
1 kit
view
MemBrite™ Fix 680/700 Cell Surface Staining Kit, 100 Reactions BT30099-T · Biotium
BT30099-T
Biotium
1 kit £176.00
1 kit
view