MemBrite™ Fix Cell Surface Staining Kits from Biotium use new covalent cell surface stains, which react rapidly and irreversibly with cell surface proteins. The stains can withstand formaldehyde or alcohol fixation and detergent permeabilisation.
Benefits of using the MemBrite™ Fix Cell Surface Staining Kits
• Quick procedure
• Uniform live/ fixed cell surface staining
• Covalent labelling can be fixed and permeabilised for IF staining
• Stain yeast and Gram-positive bacteria
• Choose from eight bright and photostable dye colours from blue to near-IR
• Trial sizes available
MemBrite™ Fix has excellent tolerance for methanol fixation
Cells were labelled with the indicated dye for five minutes at 37°C, then fixed with ice-cold methanol for five minutes at -20°C.
MemBrite™ Fix labels the cell surface rapidly and specifically, and tolerates fixation and permeabilisation
HeLa cells were labelled with MemBrite™ Fix 640/660 for five minutes at 37°C or 30 minutes at 4°C. Cells were then fixed with 4% paraformaldehyde, followed by permeabilisation with Triton X-100, or fixed with ice cold methanol. Cells labelled at 4°C were also fixed and permeabilised at 4°C.
Material available for download
Membrane and Cell Surface Stains Brochure
Note: product availability depends on country - see product detail page.