LoopSeq™ Transcriptome library preparation kits enable the generation of long read data from short read Illumina® sequencers with no extra hardware required. Using a set of enzymes to propagate a barcode intramolecularly through any piece of DNA or RNA, the LoopSeq kits use a simple, single tube workflow to eliminate PCR and GC bias as molecules are labelled in the first step and only sequenced/reported once. With significantly reduced error rates compared to those of PacBio® and Oxford Nanopore®, LoopSeq Transcriptome kits accurately determine not only transcript abundance but also unique isoforms.
Benefits of using these LoopSeq kits
• Simple, single tube workflow
• Highly accurate long-read sequencing
• Unique molecular identifier (UMI)-based transcript counting – barcode based quantification with no PCR bias
1. Get the kit
2. Upload results - securely upload FASTQ results to Loop's cloud pipeline
3. Receive data - download FASTQ and CSV files with classification and quantification data
LoopSeq technology uses single-molecule counting, which makes it possible to calculate true transcript abundance using barcode based quantification with no PCR bias.
Comparison of Loop Genomics technology vs. Illumina® technology using an ERCC control sample
TruSeq® (left) and LoopSeq Transcriptome kit (right) comparison. TruSeq® provides short reads and quantification via coverage whereas LoopSeq provides synthetic long reads from short reads with single-molecule quantification. Data shows that the LoopSeq Transcriptome kit provides a much more accurate quantification of the synthetic ERCC synthetic control sample.
|Assay time||8.5 hours|
|Hands-on time||2.5 hours|
|Mechanism of action||Synthetic long-read sequencing|
|Multiplexing||Allows for 8 samples to be processed in one tube|
|Input quantity||10 ng of total RNA|
|System compatibility||HiSeq® 2500, HiSeq® 3000, HiSeq® 4000, NextSeq®, NovaSeq®, MiSeq®, MiniSeq®|
|Nucleic acid type||RNA|
|Method||2 x 150 Paired End (PE) sequencing|
|Specialised sample types||Eukaryotic sample|
|Technology||Assembly of synthetic long-reads from short-reads|
• Full length single molecule isoform discovery and examination of splicing
• Detection of structural variants (segmental duplications, gene loss and fusion events)
• Detect RNA editing SNPs
• UMI-based transcript counting
Illumina, HiSeq, NextSeq, MiSeq, NovaSeq, MiniSeq and TruSeq are registered trademarks of Illumina, Inc. Oxford Nanopore is a trademark of Oxford Nanopore Technologies. Pacific Biosciences is a registered trademark of Pacific Biosciences.
Note: product availability depends on country - see product detail page.