LoopSeq Bacterial Genome Assembly Kit

The LoopSeq™ Bacterial Genome Assembly Kit enables the generation of long read data from short-read Illumina® sequencers with no extra hardware required. Using a set of enzymes to propagate a barcode intramolecularly through any piece of DNA or RNA, LoopSeq kits use a simple, single tube workflow to eliminate PCR and GC bias as molecules are labelled in the first step and only sequenced/reported once. This kit is ideal for de novo bacterial genome assembly and researchers wanting to obtain single-molecule long-read metagenomics data from DNA samples.

Learn more about Loop Genomics technology

Benefits of using the LoopSeq Bacterial Genome Assembly Kit
• Fewer, longer contigs
• More complete assemblies
• Single-molecule long-read data
• UMI based DNA counting – barcode based quantification with no PCR bias

LoopSeq workflow

​LoopSeq Service

1. Get the kit
2. Upload results - Securely upload FASTQ results to Loop's cloud pipeline
3. Receive data - download FASTQ and CSV files with classification and quantification data

LoopSeq Workflow

LoopSeq improves bacterial genome assembly & sequence accuracy

LoopSeq improves bacterial genome assembly & sequence accuracy

Regardless of GC content or genome size, the LoopSeq Bacterial Genome Assembly Kit consistently outperforms conventional short-read approaches

Regardless of GC content or genome size, the LoopSeq Genome Kit consistently outperforms conventional short-read approaches.

LoopSeq contigs

Left: LoopSeq reads assemble into 89- to 6-fold fewer contigs than PE150 reads or PE300 reads. Right: The largest contig assembled from LoopSeq reads averages 2.6-fold larger than the largest contig assembled from PE150 or PE300 reads.

LoopSeq comprehensive genome coverage

Left: LoopSeq assemblies average a 5.7-fold larger N50 value compared to PE150 assemblies, indicating more comprehensive genome coverage. Right: LoopSeq assemblies average a 6.2-fold lower LGA50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.

Assay time 8.5 hours
Hands-on time 2.5 hours
Mechanism of action Synthetic long read sequencing
Multiplexing Allows for 24 samples to be processed in one tube
Input quantity 10 ng genomic DNA (2 ng/µl)
Category Any linear dsDNA input
System compatibility HiSeq® 2500, HiSeq® 3000, HiSeq® 4000, NextSeq®, NovaSeq®, MiSeq®, MiniSeq®
Nucleic acid type DNA
Method 2 x 150 Paired End (PE) sequencing
Specialised sample types PCR products, genomes, metagenomes, synthetic DNA libraries, viruses
Technology Assembly of synthetic long reads from short reads


Applications
• Sequencing long range PCR products
• Targeted genomic sequencing to resolve clonal heterogeneity and phase ambiguities
• Gene panels and hybridisation capture libraries
• Synthetic biology libraries

Material available for download
LoopSeq Bacterial Genome Assembly Kit quick start guide
LoopSeq Bacterial Genome Assembly Kit user manual
Webinar: Bacterial genome sequencing and assembly with LoopSeq Bacterial Genome Kit

Illumina, HiSeq, NextSeq, MiSeq, NovaSeq, MiniSeq and TruSeq are registered trademarks of Illumina, Inc

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
LoopSeq™ Bacterial Genome Assembly Kit LGCDNA8X3RXN · Loop Genomics
LGCDNA8X3RXN
Loop Genomics
24 reactions £1730.00
24 reactions
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