The Lenti-Labeler™ vectors from System Biosciences provide an easy way to label cells in vitro for downstream in vivo applications.The Lenti-Labeler™ comes with a range of selection markers (Puro and Blasticidin), reporter genes (GFP, RFP, BFP and luciferase) and two different promoters (CMV and EF1a). With their sequences verified, all of the Lenti-Labeler™ vectors are based on time-tested and proven third generation backbones to deliver high quality and performance for nearly all potential cell labelling applications.
Benefits of using the Lenti-Labeler™
• Third generation lentivector technology for optimal virus titres
• Sequence-verified from LTR to LTR and expression-verified
• Flexible
The pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct expresses copepod GFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and coexpresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagable, sequence-verified plasmid DNA (Cat.# LL100A-1) or ready-to-transduce prepackaged lentivirus (Cat.# LL100VA-1), the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.
Available Lenti-Labeler™ constructs
Cat number | Construct | Promoter | Reporter | Selection |
---|---|---|---|---|
LL100PA-1/LL100VA-1 | pLL-CMV-GFP-T2A-Puro | CMV | GFP | Puromycin |
LL105PA-1/LL105VA-1 | pLL-CMV-GFP-T2A-Blast | CMV | GFP | Blasticidin |
LL110PA-1/LL110VA-1 | pLL-CMV-RFP-T2A-Puro | CMV | RFP | Puromycin |
LL115PA-1/LL115VA-1 | pLL-CMV-RFP-T2A-Blast | CMV | RFP | Blasticidin |
LL120PA-1/LL120VA-1 | pLL-CMV-BFP-T2A-Puro | CMV | BFP | Puromycin |
LL125PA-1/LL125VA-1 | pLL-CMV-BFP-T2A-Blast | CMV | BFP | Blasticidin |
LL150PA-1/LL150VA-1 | pLL-CMV-Luciferase-T2A-Puro | CMV | Luciferase | Puromycin |
LL200PA-1/LL200VA-1 | pLL-EF1α-GFP-T2A-Puro | EF1α | GFP | Puromycin |
LL205PA-1/LL205VA-1 | pLL-EF1α-GFP-T2A-Blast | EF1α | GFP | Blasticidin |
LL210PA-1/LL210VA-1 | pLL-EF1α-RFP-T2A-Puro | EF1α | RFP | Puromycin |
LL215PA-1/LL215VA-1 | pLL-EF1α-RFP-T2A-Blast | EF1α | RFP | Blasticidin |
LL220PA-1/LL220VA-1 | pLL-EF1α-BFP-T2A-Puro | EF1α | BFP | Puromycin |
LL225PA-1/LL225VA-1 | pLL-EF1α-BFP-T2A-Blast | EF1α | BFP | Blasticidin |
LL250PA-1/LL250VA-1 | pLL-EF1α-Luciferase-T2A-Puro | EF1α | Luciferase | Puromycin |
Lenti-Labeler™ constructs reliably and efficiently label cells
Comparison of the number of fluorescently-labelled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labelling efficiency of the Lenti-Labeler™ constructs.
While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct is compatible with most second and third generation packaging plasmid mixes, we recommend the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titres and infection of target cells.
Material available for downloadLenti-Labeler™ user manual
Products
Note: product availability depends on country - see product detail page.
Details | Cat number & supplier | Size | Price |
pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM plasmid] LL100PA-1 · System Biosciences | LL100PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-GFP-T2A-Blast [Lenti-LabelerTM plasmid] LL105PA-1 · System Biosciences | LL105PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-RFP-T2A-Puro [Lenti-LabelerTM plasmid] LL110PA-1 · System Biosciences | LL110PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-RFP-T2A-Blast [Lenti-LabelerTM plasmid] LL115PA-1 · System Biosciences | LL115PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-BFP-T2A-Puro [Lenti-LabelerTM plasmid] LL120PA-1 · System Biosciences | LL120PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-BFP-T2A-Blast [Lenti-LabelerTM plasmid] LL125PA-1 · System Biosciences | LL125PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] LL150PA-1 · System Biosciences | LL150PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-GFP-T2A-Puro [Lenti-LabelerTM plasmid] LL200PA-1 · System Biosciences | LL200PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-GFP-T2A-Blast [Lenti-LabelerTM plasmid] LL205PA-1 · System Biosciences | LL205PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid] LL210PA-1 · System Biosciences | LL210PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM plasmid] LL215PA-1 · System Biosciences | LL215PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-BFP-T2A-Puro [Lenti-LabelerTM plasmid] LL220PA-1 · System Biosciences | LL220PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-BFP-T2A-Blast [Lenti-LabelerTM plasmid] LL225PA-1 · System Biosciences | LL225PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] LL250PA-1 · System Biosciences | LL250PA-1 System Biosciences |
10 µg |
£569.00
10 µg
view
|
pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus] LL100VA-1 · System Biosciences | LL100VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-GFP-T2A-Blast [Lenti-LabelerTM virus] LL105VA-1 · System Biosciences | LL105VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-RFP-T2A-Puro [Lenti-LabelerTM virus] LL110VA-1 · System Biosciences | LL110VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-RFP-T2A-Blast [Lenti-LabelerTM virus] LL115VA-1 · System Biosciences | LL115VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-BFP-T2A-Puro [Lenti-LabelerTM virus] LL120VA-1 · System Biosciences | LL120VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-BFP-T2A-Blast [Lenti-LabelerTM virus] LL125VA-1 · System Biosciences | LL125VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM virus] LL150VA-1 · System Biosciences | LL150VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-EF1a-GFP-T2A-Puro [Lenti-LabelerTM virus] LL200VA-1 · System Biosciences | LL200VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-EF1a-GFP-T2A-Blast [Lenti-LabelerTM virus] LL205VA-1 · System Biosciences | LL205VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus] LL210VA-1 · System Biosciences | LL210VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|
pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] LL215VA-1 · System Biosciences | LL215VA-1 System Biosciences |
>2 million IFU |
£569.00
>2 million IFU
view
|