Lentiviral plasmid & prepackaged virus reagents – Lenti-Labeler™

The Lenti-Labeler™ vectors from System Biosciences provide an easy way to label cells in vitro for downstream in vivo applications.The Lenti-Labeler™ comes with a range of selection markers (Puro and Blasticidin), reporter genes (GFP, RFP, BFP and luciferase) and two different promoters (CMV and EF1a). With their sequences verified, all of the Lenti-Labeler™ vectors are based on time-tested and proven third generation backbones to deliver high quality and performance for nearly all potential cell labelling applications.

Benefits of using the Lenti-Labeler™
• Third generation lentivector technology for optimal virus titres
• Sequence-verified from LTR to LTR and expression-verified
• Flexible

Lenti-Labeler construct

The pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct expresses copepod GFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and coexpresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagable, sequence-verified plasmid DNA (Cat.# LL100A-1) or ready-to-transduce prepackaged lentivirus (Cat.# LL100VA-1), the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.

Available Lenti-Labeler™ constructs

Cat number	Construct	Promoter	Reporter	Selection
LL100PA-1/LL100VA-1	pLL-CMV-GFP-T2A-Puro	CMV	GFP	Puromycin
LL105PA-1/LL105VA-1	pLL-CMV-GFP-T2A-Blast	CMV	GFP	Blasticidin
LL110PA-1/LL110VA-1	pLL-CMV-RFP-T2A-Puro	CMV	RFP	Puromycin
LL115PA-1/LL115VA-1	pLL-CMV-RFP-T2A-Blast	CMV	RFP	Blasticidin
LL120PA-1/LL120VA-1	pLL-CMV-BFP-T2A-Puro	CMV	BFP	Puromycin
LL125PA-1/LL125VA-1	pLL-CMV-BFP-T2A-Blast	CMV	BFP	Blasticidin
LL150PA-1/LL150VA-1	pLL-CMV-Luciferase-T2A-Puro	CMV	Luciferase	Puromycin
LL200PA-1/LL200VA-1	pLL-EF1α-GFP-T2A-Puro	EF1α	GFP	Puromycin
LL205PA-1/LL205VA-1	pLL-EF1α-GFP-T2A-Blast	EF1α	GFP	Blasticidin
LL210PA-1/LL210VA-1	pLL-EF1α-RFP-T2A-Puro	EF1α	RFP	Puromycin
LL215PA-1/LL215VA-1	pLL-EF1α-RFP-T2A-Blast	EF1α	RFP	Blasticidin
LL220PA-1/LL220VA-1	pLL-EF1α-BFP-T2A-Puro	EF1α	BFP	Puromycin
LL225PA-1/LL225VA-1	pLL-EF1α-BFP-T2A-Blast	EF1α	BFP	Blasticidin
LL250PA-1/LL250VA-1	pLL-EF1α-Luciferase-T2A-Puro	EF1α	Luciferase	Puromycin

Lenti-Labeler™ constructs reliably and efficiently label cells

Comparison of the number of fluorescently-labelled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labelling efficiency of the Lenti-Labeler™ constructs.

While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct is compatible with most second and third generation packaging plasmid mixes, we recommend the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titres and infection of target cells.

Material available for download
Lenti-Labeler™ user manual

Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM plasmid] LL100PA-1 · System Biosciences	LL100PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-GFP-T2A-Blast [Lenti-LabelerTM plasmid] LL105PA-1 · System Biosciences	LL105PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-RFP-T2A-Puro [Lenti-LabelerTM plasmid] LL110PA-1 · System Biosciences	LL110PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-RFP-T2A-Blast [Lenti-LabelerTM plasmid] LL115PA-1 · System Biosciences	LL115PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-BFP-T2A-Puro [Lenti-LabelerTM plasmid] LL120PA-1 · System Biosciences	LL120PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-BFP-T2A-Blast [Lenti-LabelerTM plasmid] LL125PA-1 · System Biosciences	LL125PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] LL150PA-1 · System Biosciences	LL150PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-GFP-T2A-Puro [Lenti-LabelerTM plasmid] LL200PA-1 · System Biosciences	LL200PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-GFP-T2A-Blast [Lenti-LabelerTM plasmid] LL205PA-1 · System Biosciences	LL205PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid] LL210PA-1 · System Biosciences	LL210PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM plasmid] LL215PA-1 · System Biosciences	LL215PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-BFP-T2A-Puro [Lenti-LabelerTM plasmid] LL220PA-1 · System Biosciences	LL220PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-BFP-T2A-Blast [Lenti-LabelerTM plasmid] LL225PA-1 · System Biosciences	LL225PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-EF1a-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] LL250PA-1 · System Biosciences	LL250PA-1 System Biosciences	10 µg	£557.00 10 µg view
pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus] LL100VA-1 · System Biosciences	LL100VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-GFP-T2A-Blast [Lenti-LabelerTM virus] LL105VA-1 · System Biosciences	LL105VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-RFP-T2A-Puro [Lenti-LabelerTM virus] LL110VA-1 · System Biosciences	LL110VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-RFP-T2A-Blast [Lenti-LabelerTM virus] LL115VA-1 · System Biosciences	LL115VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-BFP-T2A-Puro [Lenti-LabelerTM virus] LL120VA-1 · System Biosciences	LL120VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-BFP-T2A-Blast [Lenti-LabelerTM virus] LL125VA-1 · System Biosciences	LL125VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM virus] LL150VA-1 · System Biosciences	LL150VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-EF1a-GFP-T2A-Puro [Lenti-LabelerTM virus] LL200VA-1 · System Biosciences	LL200VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-EF1a-GFP-T2A-Blast [Lenti-LabelerTM virus] LL205VA-1 · System Biosciences	LL205VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus] LL210VA-1 · System Biosciences	LL210VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view
pLL-EF1a-RFP-T2A-Blast [Lenti-LabelerTM virus] LL215VA-1 · System Biosciences	LL215VA-1 System Biosciences	>2 million IFU	£557.00 >2 million IFU view