Cambridge Bioscience offers an entire suite of products for the packaging, concentration and titering of pseudoviral particles. Utilising third-generation lentivector systems, LentiSuite™ provides the necessary tools for the processing of infectious particles ready for transduction into target cells for stable integration and expression.
Benefits of using the LentiSuite™ Toolkit
• Less than three hours to determine virus titers
• High titer enabled (>10^9 IFU/ml)
• Ideal for hard to transfect cells
• Mammalian cell compatible
• Replicating and nondividing cell compatible
• Third generation FIV or HIV based systems available
LentiSuite™ Toolkit
The LentiSuite™ Toolkit contains the following components for lentiviral packaging, concentration and titering:
1. Packaging: pPACK packaging plasmid mix
Provides the structural and replication proteins necessary to generate pantropic VSV-G-pseudotyped lentiviral particles. pPACK plasmids are co-transfected alongside the researcher's own lentiviral construct into a 293TN producer cell line (included in the LentiSuite™ kit) optimised for lentiviral particle production.
2. Virus concentration: PEG-it™ concentration solution (5ml)
Concentrates lentivirus particles to a concentration necessary for transduction, concentrating lentiviral particles 10-100 fold. No specialist equipment required.
Find out more here
3. Titering: Global UltraRapid™ titering kit
Enables the rapid measurement of copy numbers of integrated lentiviral constructs in the genomic DNA of transduced cells. The kit contains all the necessary reagents to quantify endogenous UCR1 elements in both mouse and human genomes and the pseudo-lentiviral specific WPRE element in less than three hours.
Find out more here
Benefits of using the LentiSuite™ Toolkit
• Less than three hours to determine virus titers
• High titer enabled (>10^9 IFU/ml)
• Ideal for hard to transfect cells
• Mammalian cell compatible
• Replicating and nondividing cell compatible
• Third generation FIV or HIV based systems available
LentiSuite™ Toolkit
The LentiSuite™ Toolkit contains the following components for lentiviral packaging, concentration and titering:
1. Packaging: pPACK packaging plasmid mix
Provides the structural and replication proteins necessary to generate pantropic VSV-G-pseudotyped lentiviral particles. pPACK plasmids are co-transfected alongside the researcher's own lentiviral construct into a 293TN producer cell line (included in the LentiSuite™ kit) optimised for lentiviral particle production.
2. Virus concentration: PEG-it™ concentration solution (5ml)
Concentrates lentivirus particles to a concentration necessary for transduction, concentrating lentiviral particles 10-100 fold. No specialist equipment required.
Find out more here
3. Titering: Global UltraRapid™ titering kit
Enables the rapid measurement of copy numbers of integrated lentiviral constructs in the genomic DNA of transduced cells. The kit contains all the necessary reagents to quantify endogenous UCR1 elements in both mouse and human genomes and the pseudo-lentiviral specific WPRE element in less than three hours.
Find out more here
Material available for download
LentiSuite™ Toolkit flyer
LentiSuite™ Toolkit citations
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps LV340A-1 · System Biosciences | LV340A-1 System Biosciences |
10 preparations |
£963.00
10 preparations
view
|
| LentiSuite Deluxe Kit (includes HEK293TN Producer Cells, pPACKH1, PureFection, PEG-it, TransDux MAX, & Global UltraRapid Titering Kit) 10 preps LV350A-1 · System Biosciences | LV350A-1 System Biosciences |
10 preparations |
£1544.00
10 preparations
view
|