Intracellular Nitric Oxide Assay Kit

Cambridge Bioscience offers a kit for the rapid and direct detection of intracellular NO or NOS activity in intact cultured cells. Using the most specific NOS specific inhibitor (L-NNA) available, the OxiSelect™ intracellular nitric oxide assay is NO specific, highly sensitive (3 nM) wih a probe suitable for flow cytometry, fluorescent microscopy and fluorescent microplate detection.

Benefits of Using the OxiSelect™ Intracellular Nitric Oxide Assay Kit
• Sensitive - detection limit of ~3 nM
• NO specific
• High photostability
• Excellent pH stability & range
• Flexible - detected with standard fluorescein filters
• Wide instrument compatibility

OxiSelect™ Intracellular Nitric Oxide Assay Kit Principle
The OxiSelect™ intracellular nitric oxide assay kit utilises a cell-permeant NO probe that passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidises to a highly fluorescent, triazolo-fluorescein analogue. The fluorescence intensity is proportional to the NO levels within the cell cytosol.Intracellular NO Assay Principle
Material Available for Download
OxiSelect™ Intracellular Nitric Oxide (NO) Assay Kit Manual

OxiSelect™ Intracellular Nitric Oxide Assay Kit Citation List

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
OxiSelectâ„¢ Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) STA-800 · Cell Biolabs
STA-800
Cell Biolabs
96 assays £510.00
96 assays
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OxiSelectâ„¢ Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) STA-800-5 · Cell Biolabs
STA-800-5
Cell Biolabs
5 x 96 assays £2169.00
5 x 96 assays
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