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  2. In-situ proximity ligation: NaveniFlex

In-situ proximity ligation: NaveniFlex

The Naveni in situ proximity ligation technology enables visualisation of protein-protein interactions, post-translational modifications or extremely low abundant proteins in cultured cells, fresh-frozen and FFPE samples. The technology uses two primary antibodies to achieve clear target detection, increased specificity and excellent consistency in staining.

Find out more about Naveni proximity ligation technology

Benefits of using the NaveniFlex kits
• Clear target detection
• Consistency in staining
• Simple protocol based on secondary detection using Navenibodies
• Compatible with traditional in situ equipment and workflows
• Sensitive fluorescent readout
• Excellent reproducibility
• Possibility to detect extremely low abundant proteins
• Detection of protein-protein interactions
• Specific detection of post-translational modifications, also with the use of pan-specific antibodies
• Each kit contains Ato488, TEX615 and Atto647N fluorophores

NaveniFlex MR

NaveniFlex MR

Left: NaveniFlex MR was used for the detection of protein interaction of beta-catenin and e-cadherin in MCF7 cells using Atto647N fluorophore. Right: NaveniFlex MR was used for the detection of protein interaction of beta-catenin and e-cadherin in human skin tissue using ATTO488 fluorophore.

NaveniFlex MM

NaveniFlex MR

Left: NaveniFlex MM was used for the detection of CA125f in CA-125, 5 + Control Slide, (Sigma Aldrich) using TEX615 fluorophore. Right: NaveniFlex MR was used for the detection of protein interaction of chromatin interactions (histone H3) with the nuclear lamina (lamin-B1) in BT474 (breast cancer cells) using TEX615 fluorophore.

Material available for download
Application note: Detection of interactions between glycoproteins in ovarian cancer using Navinci universal
Application note: Navigating chromatin organisation with NaveniFlex

Frequently asked questions

The NaveniFlex kits contain secondary detection reagents, called Navenibodies, which are carefully selected antibodies conjugated to proprietary oligo arms. Navenibodies an be used with one or two primary IgG antibodies, raised in mouse, rabbit or goat. Three fluorophore alternatives are included, allowing you to chose the most suiable fluorophore for your application.
If you have access to two primary antibodies, generated in rabbit, mouse or goat respectively, then the NaveniFlex assay is available in a variety of formats for your corresponding primary antibody pair.
SignalSlide is a registered trademark of Cell Signaling Technology, Inc.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
NaveniFlex 100 MR NF.MR.100 · Navinci Diagnostics AB
NF.MR.100
Navinci Diagnostics AB		 100 reactions £1273.00
100 reactions
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NaveniFlex 100 MM NF.MM.100 · Navinci Diagnostics AB
NF.MM.100
Navinci Diagnostics AB		 100 reactions £1273.00
100 reactions
view
NaveniFlex 100 RR NF.RR.100 · Navinci Diagnostics AB
NF.RR.100
Navinci Diagnostics AB		 100 reactions £1273.00
100 reactions
view
NaveniFlex 100 GM NF.GM.100 · Navinci Diagnostics AB
NF.GM.100
Navinci Diagnostics AB		 100 reactions £1273.00
100 reactions
view
NaveniFlex 100 GR NF.GR.100 · Navinci Diagnostics AB
NF.GR.100
Navinci Diagnostics AB		 100 reactions £1273.00
100 reactions
view

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