Arthur™ from NanoEn Tek is a fully automated, multi-channel, image-based, cytometer. Arthur includes state-of-the-art optics and algorithms for accurate brightfield and fluorescence counting. Maximise your application potential and simplify cellular analysis with the all-in-one Arthur cytometry system.
Benefits of using Arthur
• Accurate assay results
• Multiple applications with three channel acquisition
• Results in less than one minute
• User friendly with rapid setup and intuitive interface
• Export data to CSV, FCS, JPEG and PDF
Cell counting and viability
Head shocked cells(proteins) were stained with Propidium Iodide (PI) and percent of viable cells of in the total population were analysed by Arthur(A) and competitor's product F (B) with two different cell types, Jurkat and U-2 OS. In all cases, the fluorescence threshold setting was confirmed visually in the image of the Arthur. For each cell line, viability results from the Arthur were consistent with the results obtained from the competitor's product F.
Fluorescence expression (GFP and RFP)
Arthur counts and analyses cells in GFP, RFP and brightfield channels, displaying them as a histogram and circling them on the recorded image. This recording method allows easy gating based on size and fluorescence.
U-2 OS cells were transfected using the Neon™ transfection system and 0.25 μg of the five different GFP constructs and then analysed on Arthur and competitor's product F to determine transfection efficiency. The histograms in panels A (Arthur) and C (competitor's product F) show the green fluorescence intensities and counts for the transfected populations. The percentages of GFP-expressing cells detected with Arthur™ were consistent with the results from the competitor's product F.
Arthur can easily distinguish between live, dead and apoptotic cells using Annexin V (488 nm) and PI staining, with results comparable to FACS. We are able to provide accompanying annexin/PI kits for use in this application (Cat #: CAY600300-100 & BT30065).
Cell cycle analysis
The fluorescence intensity of stained cells can be detected and cell populations can be accurately gated with Arthur. The results can be used to determine cell cycle stages and automatically obtain histograms and tabulated cell population data.
Comparison of cell cycle profiles in two platforms, Arthur and competitor's Product F. HeLa cells were treated with Nocodazole (Microtubule-Destabilizer) or Epothilone B (Microtubule-Stabilizer) to arrest cells in G2/M phase. Cells were fixed with 70% ethanol and stained with Propidium Iodide (PI). The histograms show the percentages of G0/G1 phase, S phase and G2/M phase of cell cycle analysis for drug treated HeLa cells on Arthur (A) and competitor's product F (B). The cell cycle analysis of Arthur was highly consistent with the results obtained from competitor's product F
|Counting time||10 seconds - 2 minutes|
|Cell density range||1 x 105 - 1 x 107 cells/ml|
|Cell size range||5 - 60 µm|
|Sample volume||25 µl|
|Optics||GFP, RFP and Brightfield channels|
|Filers||GFP: Ex 466/40, Di 495 LP, Em 525/50
RFP: Ex 543/22, Di 580 LP, Em 585 LP
|Camera||1.3 MP, 4x objective, 4x or 16 x Digital Zoom|
|USB||4 GB supplied|
|Dimensions||290 mm (W) x 440 mm (D) x 290 mm (H)|
|Weight||8.7 kg (19.4 lbs)|
|Output format||Data: CSV, FCS
|Operating environment||5 - 40 °C, 20- 95%|
Neon is a trademark of Thermo Fisher Scientific
Note: product availability depends on country - see product detail page.
|Details||Cat number & supplier||Size||Price|
|Arthur AT1000 · NanoEnTek||
|Arthur Calibration Bead AB0100 · NanoEnTek||
|Annexin V FITC Assay Kit CAY600300-100 tests · Cayman Chemical||
CAY600300-100 testsCayman Chemical
|Apoptosis and Necrosis Quantitation Kit Plus BT30065 · Biotium||