Benefits of using the His-Spin Protein Miniprep™
• Five minute protocol
• Easy to use
• Only requires a benchtop microcentrifuge
• Purified protein can be used directly in a variety of downstream applications
Purification of 6X His-fusion proteins. E. coli cell extracts, containing indicated proteins (i.e., 112, 32, 18 kDa) expressed as a N-terminal 6X His-fusion, as well as the proteins purified using His-Spin Protein Miniprep™ were analyzed by SDS-PAGE in a 15 percent (w/v) polyacrylamide gel, and stained with Coomassie Blue®. The recombinant proteins were purposely expressed to a low level to demonstrate the efficiency of the His-Spin Protein Miniprep™.
Material available for download
His-Spin Protein Miniprep™ protocol
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