Forget-Me-Not™ Universal Probe Master Mix

Cambridge Bioscience offers a high performance master mix for rapid, real-time fluorescent-probe PCR applications including quantitation and SNP genotyping. Supplied with the superior hotstart enzyme Cheetah™ Taq which can be fully activated in just 2 minutes, the Forget-Me-Not™ Universal probe master mix is suitable for all fluorescent probe-based technologies including hydrolysis (such as TaqMan® and dual-labelled BHQ® probes) and displacement probes and offers broad instrument compatibility.

Benefits of Using the Forget-Me-Not™ Universal Probe Master Mix
• High performance
• 2 minute activation time
• Compatible with all fluorescent probe-based technologies
• Excellent activity recovery
• pH compatibility for optimal Taq activity
• Specific target amplification
• Broad instrument compatibility including Fluidigm® microfluidic PCR systems
• Helps eliminate pipetting mistakes - includes 2 colour reaction tracking buffer (optional)
• Available with or without ROX

Example Data - SNP Genotyping With Forget-Me-Not™ Universal Probe Master Mix

SNP genotyping using dual-labeled BHQ1Plus® hydrolysis probes. DNA samples were genotyped in reactions containing 450 nM of each primer and 100 nM of each probe (Allele 1 probe-FAM/ BHQ®-1, Allele 2 probe- CAL Fluor® Orange 560/BHQ®-1) on a QuantStudio® 5 system (ThermoFisher Scientific).

Material Available for Download
2X Forget-Me-Not™ Universal Probe Master Mix Protocol

Fluidigm is a registered trademark of Fluidigm Corporation
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
BHQ1Plus & CAL Fluor are a registered trademarks of Biosearch Technologies, Inc.
QuantStudio is a registered trademark of Life Technologies Corp.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Forget-Me-Not™ Universal Probe Master Mix (100 reactions) BT31043-T · Biotium
BT31043-T
Biotium
1 kit £67.00
1 kit
view
Forget-Me-Not™ Universal Probe Master Mix (500 reactions) BT31043-1 · Biotium
BT31043-1
Biotium
1 kit £286.00
1 kit
view
Forget-Me-Not™ Universal Probe Master Mix with ROX (100 reactions) BT31044-T · Biotium
BT31044-T
Biotium
1 kit £67.00
1 kit
view
Forget-Me-Not™ Universal Probe Master Mix with ROX (500 reactions) BT31044-1 · Biotium
BT31044-1
Biotium
1 kit £286.00
1 kit
view