Cambridge Bioscience offers a comprehensive range of expression vectors for the self made production of expression constructs including pCMV bacterial based vectors and protein expression systems.
Our portfolio includes pCMV vectors from our supplier OriGene. These vectors contain a CMV promoter, which can be used to express the cloned cDNA and a hGH (human growth hormone) polyA signal located downstream of the insert. These vectors contain multiple origins of replication including ColE1 ori, SV40 ori allowing replication in mammalian cells and f1 ori (filamentous phage ori) allowing for the recovery of single-stranded plasmids. In addition to this, our portfolio contains a range of precision shuttling vectors and kits to accommodate diverse tagging needs enabling easy subcloning of an ORF from one tagged vector to another.
Our portfolio includes the Expresso Systems from Lucigen. This system offers enzyme-free directional PCR cloning in seconds including cloning-ready vectors and competent cell lines. This syestem enables tightly controlled expression of N- or C-terminal 6xHis tagged proteins and to quickly purify expressed proteins by metal affinity chromatography.
Note: product availability depends on country - see product detail page.