www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

DNA/RNA Shield Lysis Tubes

The DNA/RNA Shield™ Lysis Tubes from Zymo Research ensure sample stability during storage/transport at ambient temperatures, without the need for refrigeration or specialised equipment.

DNA/RNA Shield and BashingBead™ technologies effectively lyse samples and inactivate pathogens (e.g., virus, bacteria). Nucleic acids are preserved at ambient temperature (DNA>1 year; RNA up to 1 month), and samples in DNA/RNA Shield can be frozen (-20/-80°C) for prolonged storage.

Benefits of using the DNA/RNA Shield Lysis TubesDNA/RNA Shield Lysis Tube
• No reagent removal required prior to nucleic acid purification
• Tube prefilled with ultra-high density Bashingbeads™ for homogenisation
• RNA stable at room temperature for one month
• DNA stable at room temperature for two years
• Available with/without nylon swab

DNA/RNA Shield Lysis Tube workflow

DNA/RNA Shield Lysis Tube workflow

DNA/RNA Shield product
Catalogue number         
Description
DNA/RNA Shield Reagent R1100-50
R1100-250
All-purpose reagent for sample lysis and stabilisation of DNA/RNA in tissues (e.g. mammalian biopsy, whole insects), cells (animal, microbial) and biological liquids.
2X concentrate R1200-25
R1200-125
Reagent concentrate (2X) for use with biological liquids at 1:1 ratio.
(Note: (2X) concentrate can be diluted to (1X) solution with nuclease-free water)
Lysis Tube (Microbe) R1103
R1104 (w/ swab)
2 ml collection tube (prefilled with 1 ml DNA/RNA Shield) and Microbe Bashing Beads for the collection and homogenisation of microbial samples.
Lysis Tube (Tissue) R1105 2 ml collection tube (prefilled with 1 ml DNA/RNA Shield) and Tissue Bashing Beads for the collection and homogenisation of tissue samples.
Collection Tube R1102 2 ml collection tube (prefilled with 1 ml DNA/RNA Shield) for collection of any sample.
Blood Collection Tube R1150 16 x 100 mm evacuated blood tube (prefilled with 6 ml DNA/RNA Shield) for the direct collection of 3 ml whole blood (human or animal)
Fecal Collection Tube R1101 15 ml container (prefilled with 9 ml DNA/RNA Shield) for the direct collection of up to 1 gram or 1 ml stool. Collection spoon is included in the container screwcap.
Collection Tube w/ Swab                                                                 R1106 (10 pack)
R1107 (50 pack)                                     
12 x 80 mm screwcap container filled with 1 ml DNA/RNA Shield and a sterile swab for specimen collection.


DNA/RNA Shield can be added to frozen or fresh samples to prevent degradation

Add DNA/RNA Shield frozen or fresh samples to prevent degradation

Left: High-quality RNA from blood stored in DNA/RNA Shield that was freeze-thawed from -80°C to room temperature. Whole blood samples +/- DNA/RNA Shield were subjected to > 2 freeze thaw cycles. Total RNA was subsequently purified using the Quick-RNA™ Whole Blood Miniprep Kit. Right: High-quality DNA from stool stored in DNA/RNA Shield after up to 10 freeze-thaw cycles. Microbial composition profiling via 16S rRNA gene targeted sequencing.

DNA/RNA Shield: complete pathogen inactivation
DNA/RNA Shield has been rigorously tested to ensure its capability to inactive even the toughest of viruses. In an independent study, the virucidal activity was shown to inactivate murine parvovirus1. DNA/RNA Shield abides by the Center for Disease Control’s (CDC) guidelines for pathogen inactivation2.

DNA/ RNA Shield effectively inactivates pathogens. This includes tough-to-lyse microbes or viruses without the need for additional steps, such as heat-treatment, homogenisation, or alcohol sterilisation. DNA/RNA Shield has been rigorously tested to ensure inactivation of pathogens including:

Bacteria 
B. subtilis, E. faecalis, E. coli, L. fermentum, L. monocytogenes, M. tuberculosis, P. aeruginosa, S. enterica, S. aureus, S. pneumoniae, X. fastidiosa

Viruses 
Parvovirus, Chikungunya virus , Dengue virus, Ebola virus, Herpes simplex virus-1, Herpes simplex virus-2, Influenza A, Rhinovirus, MERS-coronavirus, West Nile virus 

Yeast & eukaryotes 
C. albicans , C. neoformans , S. cerevisiae , P. malariae

1. Dr. Thraenhart and Dr. Jursch. Virucidal activity of the nucleic acid preservation product “DNA/RNA Shield™” against the murine parvovirus (MVM) at 20 °C. 
2. Guidance on the inactivation or removal of select agents and toxins for future use. Center for Disease Control (CDC)

Microbiomics & metagenomics portfolio
Cambridge Bioscience offers a comprehensive solution for microbiomic and metagenomic research from collection to analyses. See the full range.

Material available to download
DNA/RNA Shield Lysis Tube protocol
DNA/RNA Shield Lysis & Collection Tubes recommended purification kits

DNA/RNA Shield citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
DNA/RNA Shield Collection Tube R1102 · Zymo Research
R1102
Zymo Research
50 pack £256.00
50 pack
view
DNA/RNA Shield Lysis Tubes (microbe) R1103 · Zymo Research
R1103
Zymo Research
50 pack £330.00
50 pack
view
DNA/RNA Shield Lysis Tubes with Swab (microbe) R1104 · Zymo Research
R1104
Zymo Research
50 pack £375.00
50 pack
view
DNA/RNA Shield Lysis Tubes (tissue) R1105 · Zymo Research
R1105
Zymo Research
50 pack £330.00
50 pack
view