Cambridge Bioscience offers a kit for the rapid and reliable bisulfite treatment and complete conversion of cytosines in RNA for methylation analysis. Integrating denaturation and bisulphite conversion into a single-step the EZ RNA Methylation™ kit eliminates cumbersome precipitations, reduces template degradation and minimises DNA loss during treatment and clean-up.
Benefits of Using the EZ RNA Methylation™ Kit
• Fast & reliable bisulfite conversion
• >99% conversion efficiency
• >99% protection of 5-methylcytosine
• Excellent RNA recovery - >80%
• Streamlined process
• Template degradation & loss of RNA minimised
• Messy precipitation steps eliminated
• Wide sample compatibility
Sequencing Results Following Bisulfite Treatment
RNA with methylated C (5-mC) at nucleotide position #7 was processed using the EZ RNA methylation™ kit. Recovered RNA was amplified by RT-PCR, cloned and sequenced. The methylated cytosine at position #7 remained intact. The non-methylated cytosines at positions #3, 4, and 13 were completely converted into uracil (post-bisulfite treatment) and detected as thymine following RT-PCR and sequencing.
• Library preparation
• Next-Gen sequencing
Material Available For Download
EZ RNA Methylation™ Kit Protocol
EZ RNA Methylation™ Kit Citations
Note: product availability depends on country - see product detail page.