CF™680 & CF™680R Dyes

Cambridge Bioscience offers a highly water-soluble cyanine-based dye which produces the brightest fluorescence and highest signal-to-noise ratio in immunostaining among spectrally similar dyes. CF™680 is excellent option for labelling antibodies.

We also offer a novel rhodamine-based dye that is highly fluorescent and boasts extremely high photostability. As a result, CF™680R is ideal for confocal microscopy, single molecule-based imaging and is also preferred over CF™680 for the labelling of nucleic acids and relatively small biomolecules.

CF™680 Technical Details
• 681/698 nm Abs/Em Maxima
• 210,000 Extinction Coefficient
• ~ 3241 Molecular Weight
• 680 nm or 685 nm Excitation Laser Line
• Direct Replacement For Alexa Fluor® 680, Cy™5.5

Benefits Of Using CF™680
• Brightest among spectrally similar 680 nm dyes
• Superior signal-to-noise ratio in immunostaining
• Highly water-soluble & pH-insensitive

CF™680RTechnical Details
• 680/701 nm Abs/Em Maxima
• 140,000 Extinction Coefficient
• ~ 912 Molecular Weight
• 680 nm or 685 nm Excitation Laser Line
• Direct Replacement For Alexa Fluor® 680, Cy™5.5

Benefits Of Using CF™680R
• Most photostable 680 nm dye
• Suitable for labeling nucleic acids & small bio-molecules
• Highly water-soluble & pH-insensitive

Signal to Noise Ratio

CF680
Intracellular staining of Jurkat cells was performed using mouse anti-human CD3 antibody or isotype control followed by goat anti-mouse secondary antibody conjugates. Fluorescence was analyzed on a BD FACSCalibur flow cytometer in the FL4 channel Bars represent the signal-to-noise ratio of CD3-positive fluorescence to isotype control.

Stability Comparison
CF680 2
Photostability of far red dye conjugates. Jurkat cells were stained with mouse anti-CD3 followed by the indicated goat anti-mouse IgG conjugates. Cells were continuously exposed to mercury arc lamp excitation with a Cy™5 filter set. Images were captured every 10 seconds for 5 minutes and fluorescence intensity was normalized to time 0.

CF™680 & CF™680R Formats Available
1. Dye Labelled Secondary Antibodies
2. Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - To find out more, please Click Here
3. Conjugates

Please browse the selection below, use the search function or contact our Biotium specialist here to find the required product.

CF™ Dye Citation List

Alexa Fluor® is a registered trademark of Invitrogen
Cy™ is a trademark of GE Healthcare

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CF®680 Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20062-1 · Biotium
BT20062-1
Biotium
50 ul £89.00
50 ul
view
Mix-n-Stain™ CF®680R Antibody Labeling Kit, 1x(50-100ug) labeling BT92246 · Biotium
BT92246
Biotium
1 kit £138.00
1 kit
view
CF®680 maleimide BT92029 · Biotium
BT92029
Biotium
1 umol £244.00
1 umol
view
Mix-n-Stain™ CF®680 Antibody Labeling Kit, 1x(50-100ug) labeling BT92240 · Biotium
BT92240
Biotium
1 kit £138.00
1 kit
view
Mix-n-Stain™ CF®680R Antibody Labeling Kit,1x(20-50ug) labeling BT92263 · Biotium
BT92263
Biotium
1 kit £124.00
1 kit
view
CF®680, succinimidyl ester BT92139 · Biotium
BT92139
Biotium
1 umol £286.00
1 umol
view
CF®680 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20241 · Biotium
BT20241
Biotium
500 ul £220.00
500 ul
view
Mix-n-Stain™ CF®680 Antibody Labeling Kit,1x(20-50ug) labeling BT92262 · Biotium
BT92262
Biotium
1 kit £124.00
1 kit
view
CF®680 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20241-1 · Biotium
BT20241-1
Biotium
50 ul £89.00
50 ul
view
Mix-n-Stain™ CF®680R Antibody Labeling Kit, 1x(5-20ug) labeling BT92283 · Biotium
BT92283
Biotium
1 kit £114.00
1 kit
view
Mix-n-Stain™ CF®680 Antibody Labeling Kit, 1x(5-20ug) labeling BT92282 · Biotium
BT92282
Biotium
1 kit £114.00
1 kit
view
CF®680R Protein Labeling Kit "3 labelings" BT92226 · Biotium
BT92226
Biotium
1 kit £434.00
1 kit
view
Wheat Germ Agglutinin, CF®680R Conjugate BT29025 · Biotium
BT29025
Biotium
5 x 1 mg £338.00
5 x 1 mg
view
Phalloidin, CF®680R BT00048 · Biotium
BT00048
Biotium
300 units £425.00
300 units
view
CF®680 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20060 · Biotium
BT20060
Biotium
250 ul £210.00
250 ul
view
Wheat Germ Agglutinin, CF®680R Conjugate BT29025-1 · Biotium
BT29025-1
Biotium
1 mg £88.00
1 mg
view
CF®680 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20060-1 · Biotium
BT20060-1
Biotium
50 ul £89.00
50 ul
view
CF®680 Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20061 · Biotium
BT20061
Biotium
250 ul £210.00
250 ul
view
CF®680R maleimide BT92032 · Biotium
BT92032
Biotium
1 umol £244.00
1 umol
view
CF®680 Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20061-1 · Biotium
BT20061-1
Biotium
50 ul £89.00
50 ul
view
CF®680R, aminooxy BT92054 · Biotium
BT92054
Biotium
1 mg £512.00
1 mg
view
CF®680 Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20062 · Biotium
BT20062
Biotium
250 ul £210.00
250 ul
view
CF®680R succinimidyl ester BT92107 · Biotium
BT92107
Biotium
1 umol £298.00
1 umol
view
CF®680R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2mg/mL BT20192 · Biotium
BT20192
Biotium
500 ul £220.00
500 ul
view
CF®680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL BT20068-1 · Biotium
BT20068-1
Biotium
50 ul £84.00
50 ul
view