CF™640R: A Highly Photostable Far-Red Dye

Cambridge Bioscience offers a rhodamine-based far-red fluorescent dye which is much brighter than Cy™5, at least as bright as Alexa Fluor® 647 and with better photostability over Cy™5 and Alexa Fluor® 647. CF™640R is also much brighter and more photostable than Atto™ 647N, another spectrally similar dye frequently used in single-molecule imaging. These features combined make CF™640R ideal for confocal microscopy, single-molecule imaging & other demanding applications.

CF™640R Technical Details
• 642/662 nm Abs/Em Maxima
• 105,000 Extinction Coefficient
• ~ 832 Molecular Weight
• 633 nm, 635 nm or 640 nm Excitation Laser Line
• Direct Replacement For Alexa Fluor® 647, ATTO™ 647N, Cy™5 & DyLight™649

Benefits Of Using CF™640R
• Brighter than Cy™5
• Better photostability than Cy™5 and Alexa Fluor® 647

Brightness Comparison

CF640R
Intracellular staining of Jurkat cells was performed using mouse anti-CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analysed on a BD FACSCalibur flow cytometer in the FL4 channel. Bars represent the relative fluorescence of the geometric means of the cell populations.
CF640R
Photostability comparison between CF™640R and Alexa Fluor® 647 (AF647). HeLa cells were stained with anti-tubulin antibody conjugates of CF™640R or Alexa Fluor® 647 (AF647). Cells were continuously illuminated by a mercury arc lamp microscope and sequential images were captured at 0, 1, and 3 minutes.

CF™640R Formats Available
1. Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - please click here to find out more
2. Conjugates
3. Labelled antibodies

Please browse the selection below or contact our Biotium specialist here.

CF™ Dye Citation List

Alexa Fluor® is a registered trademark of Invitrogen
DyLight™ is a trademark of Thermo Fisher Scientific Inc.
ATTO dyes are products of ATTO-TEC GmbH
Cy™ is a trademark of GE Healthcare

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Mix-n-Stain™ CF®640R Antibody Labeling Kit, 1x(50-100ug) labeling BT92245 · Biotium
BT92245
Biotium
1 kit £138.00
1 kit
view
CF®640R-dUTP, Lyophilized Powder BT40007 · Biotium
BT40007
Biotium
25 nmol £380.00
25 nmol
view
Mix-n-Stain™ CF®640R Antibody Labeling Kit,1x(20-50ug) labeling BT92258 · Biotium
BT92258
Biotium
1 kit £124.00
1 kit
view
Mix-n-Stain™ CF®640R Antibody Labeling Kit, 1x(5-20ug) labeling BT92278 · Biotium
BT92278
Biotium
1 kit £114.00
1 kit
view
CF®640R maleimide BT92034 · Biotium
BT92034
Biotium
1 umol £244.00
1 umol
view
CF®640R amine BT92043 · Biotium
BT92043
Biotium
1 mg £227.00
1 mg
view
CF®640R aminooxy BT92058 · Biotium
BT92058
Biotium
1 mg £464.00
1 mg
view
CF®640R succinimidyl ester BT92108 · Biotium
BT92108
Biotium
1 umol £267.00
1 umol
view
CF®640R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20176-1 · Biotium
BT20176-1
Biotium
50 ul £89.00
50 ul
view
Alpha-Bungarotoxin, CF®640R BT00004 · Biotium
BT00004
Biotium
500 µg £453.00
500 µg
view
CF®640R Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20081 · Biotium
BT20081
Biotium
500 ul £215.00
500 ul
view
CF®640R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20177 · Biotium
BT20177
Biotium
500 ul £215.00
500 ul
view
CF®640R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20177-1 · Biotium
BT20177-1
Biotium
50 ul £89.00
50 ul
view
CF®640R Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20081-1 · Biotium
BT20081-1
Biotium
50 ul £89.00
50 ul
view
CF®640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL BT20085 · Biotium
BT20085
Biotium
500 ul £180.00
500 ul
view
CF®640R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20178 · Biotium
BT20178
Biotium
500 ul £215.00
500 ul
view
CF®640R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20178-1 · Biotium
BT20178-1
Biotium
50 ul £89.00
50 ul
view
CF®640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL BT20085-1 · Biotium
BT20085-1
Biotium
50 ul £72.00
50 ul
view
CF®640R Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20088 · Biotium
BT20088
Biotium
500 ul £215.00
500 ul
view
CF®640R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20179 · Biotium
BT20179
Biotium
500 ul £215.00
500 ul
view
CF®640R Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20088-1 · Biotium
BT20088-1
Biotium
50 ul £89.00
50 ul
view
CF®640R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20179-1 · Biotium
BT20179-1
Biotium
50 ul £89.00
50 ul
view
CF®640R Goat Anti-Mouse IgG (H+L), 2 mg/mL BT20197 · Biotium
BT20197
Biotium
500 ul £180.00
500 ul
view
CF®640R Goat Anti-Swine IgG (H+L), 2 mg/mL BT20089 · Biotium
BT20089
Biotium
500 ul £180.00
500 ul
view
CF®640R Goat Anti-Mouse IgG (H+L), 2 mg/mL BT20197-1 · Biotium
BT20197-1
Biotium
50 ul £72.00
50 ul
view