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CF™633: The Best Dye for 633/635 Lasers

Cambridge Bioscience offers a far-red fluorescent dye which can be optimally excited by the 633 nm He-Ne laser or the 635 nm red diode laser. Despite its shorter emission wavelength, CF™633 is still significantly brighter than Alexa Fluor® 647 and has excellent photostability.

CF™633 Technical Details
• 630/650 nm Abs/Em Maxima
• 100,000 Extinction Coefficient
• ~820 Molecular Weight
633 nm or 635 nm Excitation Laser Line
• Direct Replacement For Alexa Fluor® 633, Alexa Fluor® 647, Cy™5, DyLight™ 633, DyLight™ 649

Benefits Of Using CF™633
• Significantly brighter than Alexa Fluor® 647
• Excellent photostability

Fluorescence Intensity Comparison
CF633Intracellular staining of Jurkat cells was performed using mouse anti-CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analysed on a BD FACSCalibur flow cytometer in the FL4 channel. Bars represent the relative fluorescence of the geometric means of the cell populations.

Photostability Comparison
CF633 2Relative photostability of CF™633 and Alexa Fluor® 647 goat anti-mouse conjugates. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™633 or Alexa Fluor® 647 goat anti-rabbit IgG conjugates. Cells were imaged using a mercury arc lamp microscope equipped with a Cy™5 filter set and CCD camera. Sequential images were captured at 0, 1 and 5 minutes.

CF™633 Formats Available
1. Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - please click here to find out more
2. Conjugates
3. Labelled antibodies

Please browse the selection below or contact our Biotium specialist here.

CF™ Dye Citation List

Alexa Fluor® is a registered trademark of Invitrogen
DyLight™ is a trademark of Thermo Fisher Scientific Inc.
Cy™ is a trademark of GE Healthcare

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CF®633 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20125-1 · Biotium
BT20125-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Rabbit Anti-Chicken IgY (IgG) (H+L), 2 mg/mL BT20165 · Biotium
BT20165
Biotium
500 µl £180.00
500 µl
view
CF®633 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20121 · Biotium
BT20121
Biotium
500 µl £220.00
500 µl
view
CF®633 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20121-1 · Biotium
BT20121-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20125 · Biotium
BT20125
Biotium
500 µl £215.00
500 µl
view
CF®633 Rabbit Anti-Chicken IgY (IgG) (H+L), 2 mg/mL BT20165-1 · Biotium
BT20165-1
Biotium
50 µl £72.00
50 µl
view
CF®633 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20168 · Biotium
BT20168
Biotium
500 µl £215.00
500 µl
view
Mix-n-Stain™ CF®633 Antibody Labeling Kit, 1x(50-100ug) labeling BT92237 · Biotium
BT92237
Biotium
1 kit £138.00
1 kit
view
CF®633 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20124-1 · Biotium
BT20124-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20126 · Biotium
BT20126
Biotium
500 µl £215.00
500 µl
view
Mix-n-Stain™ CF®633 Antibody Labeling Kit,1x(20-50ug) labeling BT92257 · Biotium
BT92257
Biotium
1 kit £124.00
1 kit
view
CF®633 Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20168-1 · Biotium
BT20168-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20126-1 · Biotium
BT20126-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20171 · Biotium
BT20171
Biotium
500 µl £215.00
500 µl
view
Mix-n-Stain™ CF®633 Antibody Labeling Kit, 1x(5-20ug) labeling BT92277 · Biotium
BT92277
Biotium
1 kit £114.00
1 kit
view
CF®633 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL BT20206 · Biotium
BT20206
Biotium
250 µl £215.00
250 µl
view
CF®633 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20127 · Biotium
BT20127
Biotium
500 µl £215.00
500 µl
view
CF®633 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20127-1 · Biotium
BT20127-1
Biotium
50 µl £89.00
50 µl
view
CF®633 Monoclonal Mouse Anti-GFP IgG, 1mg/mL BT20217 · Biotium
BT20217
Biotium
100 µl £262.00
100 µl
view
CF®633 Rabbit Anti-Goat IgG (H+L), 2 mg/mL BT20128 · Biotium
BT20128
Biotium
500 µl £180.00
500 µl
view
CF®633 Chicken Anti-Rabbit IgG (H+L), 2 mg/mL BT20224 · Biotium
BT20224
Biotium
500 µl £180.00
500 µl
view
CF®633 Chicken Anti-Rabbit IgG (H+L), 2 mg/mL BT20224-1 · Biotium
BT20224-1
Biotium
50 µl £77.00
50 µl
view
Phalloidin, CF®633 BT00046 · Biotium
BT00046
Biotium
300 units £399.00
300 units
view
CF®633 Rabbit Anti-Goat IgG (H+L), 2 mg/mL BT20128-1 · Biotium
BT20128-1
Biotium
50 µl £72.00
50 µl
view
CF®633 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL BT20129 · Biotium
BT20129
Biotium
500 µl £180.00
500 µl
view