CF™543: Bright Fluorophore for the 543 nm Laser

Cambridge Bioscience offers an orange fluorescent dye based on a new class of rhodamine dyes allowing extremely bright fluorescence, good photostability and high water solubility. CF™543 yields the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes.

CF™543 Technical Details
• 541/560 nm Abs/Em maxima
• 100,000 extinction coefficient
• ~ 870 molecular weight
• 532 nm, 543 nm or 546 nm excitation laser line
• Direct replacement for Alexa Fluor® 546 & Tetramethylrhodamine (TAMRA)

Fluorescence Intensity Comparison

CF453Relative fluorescence of CF™543 and Alexa Fluor® 546 (AF546) goat anti-mouse conjugates as a function of the number of dye molecules per protein (degree of labeling).

Benefits Of Using CF™543
• Extremely bright fluorescence
• High water solubility

CF™543 Dye Formats Available
• Labelled Secondary Antibodies
• Conjugates
• Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - to find out more, please Click Here

A small selection of the CF™543 products avilable are shown below. Alternatively, please use the wbsite search to find the product of choice or contact our Biotium specialist here for more information.

CF™ Dye Citation List

Alexa Fluor® is a registered trademark of Invitrogen

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CF®543 Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2mg/mL BT20320 · Biotium
BT20320
Biotium
500 ul £215.00
500 ul
view
CF®543-dUTP, Lyophilized Powder BT40002 · Biotium
BT40002
Biotium
25 nmol £380.00
25 nmol
view
Human Transferrin, CF®543 Conjugate BT00082 · Biotium
BT00082
Biotium
1 mg £102.00
1 mg
view
CF®543 maleimide BT92044 · Biotium
BT92044
Biotium
1 umol £286.00
1 umol
view
Phalloidin, CF®543 BT00043 · Biotium
BT00043
Biotium
300 units £399.00
300 units
view
Streptavidin, CF®543 Conjugate BT29043 · Biotium
BT29043
Biotium
1 mg £214.00
1 mg
view
CF®543 succinimidyl ester BT92105 · Biotium
BT92105
Biotium
1 umol £267.00
1 umol
view
Mix-n-Stain™ CF®543 Antibody Labeling Kit, 1x(5-20ug) labeling BT92287 · Biotium
BT92287
Biotium
1 kit £114.00
1 kit
view
Mix-n-Stain™ CF®543 Antibody Labeling Kit, 1x(20-50ug) labeling BT92267 · Biotium
BT92267
Biotium
1 kit £124.00
1 kit
view
Mix-n-Stain™ CF®543 Antibody Labeling Kit, 1x(50-100ug) labeling BT92247 · Biotium
BT92247
Biotium
1 kit £138.00
1 kit
view
CF®543 Protein Labeling Kit "3 labelings" BT92209 · Biotium
BT92209
Biotium
1 kit £434.00
1 kit
view
CF®543 Chicken Anti-Goat IgG (H+L), 2 mg/mL BT20333-500uL · Biotium
BT20333-500uL
Biotium
500 ul £180.00
500 ul
view
CF®543 Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20313 · Biotium
BT20313
Biotium
500 ul £215.00
500 ul
view
CF®543 Chicken Anti-Mouse IgG (H+L), 2 mg/mL BT20334-500uL · Biotium
BT20334-500uL
Biotium
500 ul £180.00
500 ul
view
CF®543 Chicken Anti-Rabbit IgG (H+L), 2 mg/mL BT20335-500uL · Biotium
BT20335-500uL
Biotium
500 ul £180.00
500 ul
view
CF®543 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20314 · Biotium
BT20314
Biotium
500 ul £215.00
500 ul
view
CF®543 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20316 · Biotium
BT20316
Biotium
500 ul £215.00
500 ul
view
CF®543 Donkey Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2mg/mL BT20318-1 · Biotium
BT20318-1
Biotium
50 ul £89.00
50 ul
view
CF®543 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20305 · Biotium
BT20305
Biotium
500 ul £215.00
500 ul
view
CF®543 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20308 · Biotium
BT20308
Biotium
500 ul £215.00
500 ul
view
CF®543 Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2mg/mL BT20322 · Biotium
BT20322
Biotium
500 ul £215.00
500 ul
view
CF®543 Goat Anti-Chicken IgY(H+L), Highly Cross-Adsorbed, 2 mg/mL BT20311 · Biotium
BT20311
Biotium
500 ul £215.00
500 ul
view
CF®543 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL BT20317 · Biotium
BT20317
Biotium
500 ul £180.00
500 ul
view
CF®543 Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2mg/mL BT20319 · Biotium
BT20319
Biotium
500 ul £215.00
500 ul
view
CF®543 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (min X Rat), 2 mg/mL BT20328 · Biotium
BT20328
Biotium
500 ul £280.00
500 ul
view