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CF™488A: superior specific labelling

The CF™488A green fluorescent dye gives greater target specificity when bound to antibodies than those labelled with Cy™2 or Alexa Fluor® 488. CF™488A is less highly charged than Alexa Fluor® 488 therefore not distorting the protein isoelectric point and affinity, increasing the signal to noise ratio.CF™488A has similar brightness to Alexa Fluor® 488 whilst reducing spillover into the red channel.

CF™488A technical details
• 490/515 nm Abs/Em Maxima
• 70,000 extinction coefficient
• ~710 molecular weight
• 488 nm excitation laser line
• Direct replacement for Alexa Fluor® 488, DyLight™488, fluorescein (aka FITC, FAM) and Cy™2

Benefits of using CF™488A
• Antibody conjugates show increased sensitivity over competitors
• High photostability
• Water soluble and pH insensitive
• Reduced spillover into red channel compared to Alexa Fluor® 488

Staining Specificity ComparisonCF488A

Cryosections of human heart stained with rabbit anti-fibronectin followed by goat anti-rabbit IgG conjugated to Cy2, Alexa Fluor 488, and CF488A, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim, Hessen, Germany.

CF™488A formats available
• Labelled Secondary Antibodies - find out more.
• Mix-n-Stain™ CF™ Dye Antibody Labelling Kits - find out more.

A small selection of the CF™488A products avilable are shown below. Alternatively, please use the website search to find the product of choice or contact our Biotium specialist for more information.

CF™ Dye citation list

Alexa Fluor® is a registered trademark of Invitrogen. 
DyLight™ is a trademark of Thermo Fisher Scientific Inc.
Cy™ is a trademark of GE Healthcare. 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CF®488A Chicken Anti-Goat IgG (H+L), 2 mg/mL BT20225-1 · Biotium
BT20225-1
Biotium
50 µl £77.00
50 µl
view
CF®488A Chicken Anti-Rabbit IgG (H+L),2 mg/mL BT20209-1 · Biotium
BT20209-1
Biotium
50 µl £72.00
50 µl
view
CF®488A Chicken Anti-Rabbit IgG (H+L),2 mg/mL BT20209 · Biotium
BT20209
Biotium
500 µl £180.00
500 µl
view
CF®488A Chicken Anti-Goat IgG (H+L), 2 mg/mL BT20225 · Biotium
BT20225
Biotium
500 µl £180.00
500 µl
view
CF®488A Chicken Anti-Mouse IgG (H+L),2 mg/mL BT20208-1 · Biotium
BT20208-1
Biotium
50 µl £72.00
50 µl
view
CF®488A TUNEL Assay Apoptosis Detection Kit BT30063 · Biotium
BT30063
Biotium
1 kit £506.00
1 kit
view
CF®488A Protein Labeling Kit "3 labelings" BT92213 · Biotium
BT92213
Biotium
1 kit £434.00
1 kit
view
Alpha-Bungarotoxin, CF®488A BT00005 · Biotium
BT00005
Biotium
500 µg £453.00
500 µg
view
CF®488A amine BT92037 · Biotium
BT92037
Biotium
1 mg £227.00
1 mg
view
CF®488A, aminooxy BT92051 · Biotium
BT92051
Biotium
1 mg £464.00
1 mg
view
Annexin V, CF®488A Conjugate BT29005 · Biotium
BT29005
Biotium
0.5 ml £262.00
0.5 ml
view
Concanavalin A, CF®488A Conjugate BT29016 · Biotium
BT29016
Biotium
5 x 1 mg £163.00
5 x 1 mg
view
CF®488A-dUTP, Lyophilized Powder BT40008 · Biotium
BT40008
Biotium
25 nmol £380.00
25 nmol
view
CF®488A maleimide BT92022 · Biotium
BT92022
Biotium
1 umol £244.00
1 umol
view
CF®488A hydrazide BT92152 · Biotium
BT92152
Biotium
1 mg £180.00
1 mg
view
Phalloidin, CF®488A BT00042 · Biotium
BT00042
Biotium
300 units £399.00
300 units
view
Streptavidin, CF®488A Conjugate BT29034 · Biotium
BT29034
Biotium
1 mg £199.00
1 mg
view
Wheat Germ Agglutinin, CF®488A Conjugate BT29022-1 · Biotium
BT29022-1
Biotium
1 mg £75.00
1 mg
view
Wheat Germ Agglutinin, CF®488A Conjugate BT29022 · Biotium
BT29022
Biotium
5 x 1 mg £313.00
5 x 1 mg
view
CF®488A and 7-AAD Apoptosis Assay Kit BT30060 · Biotium
BT30060
Biotium
1 kit £237.00
1 kit
view
CF®488A and PI Apoptosis Assay Kit BT30061 · Biotium
BT30061
Biotium
1 kit £237.00
1 kit
view
Mix-n-Stain™ CF®488A Antibody Labeling Kit, 1x(5-20ug) labeling BT92273 · Biotium
BT92273
Biotium
1 kit £114.00
1 kit
view
Mix-n-Stain™ CF®488A Antibody Labeling Kit,1x(20-50ug) labeling BT92253 · Biotium
BT92253
Biotium
1 kit £124.00
1 kit
view
Mix-n-Stain™ CF®488A Antibody Labeling Kit, 1x(50-100ug) labeling BT92233 · Biotium
BT92233
Biotium
1 kit £138.00
1 kit
view
CF®488A Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL BT20022-1 · Biotium
BT20022-1
Biotium
50 µl £89.00
50 µl
view