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Cell Differentiation Related microRNA Profiling

Cambridge Bioscience offers a stem cell microRNA qPCR panel and the QuantiMir™  to simultaneously measure the expression of 95 miRNAs associated with stem cell renewal, haematopoiesis, neural development and tissue patterning down to picogram quantities in total RNA samples.

Benefits of Using the Stem Cell qPCR Array & QuantiMir™ System
• Simultaneously profile 95 different MicroRNAs known to be involved in stem cell self-renewal, haematopoiesis, neural development and tissue patterning
• Identify miRNA development biomarkers and expression pattern signatures in Stem Cells
• System rapidly tags and converts all small RNAs into detectable cDNA for qPCR
• Simple and robust procedure
• Sensitive and quantitative down to picogram-quantities of starting total RNA
• Compatible with high-throughput screening of numerous cell lines and tissues

Stem Cell MicroRNA qPCR Array
Supplied in a convenient 96-well plate format, segmented into 4 separate stem cell pathways, this array contains 95 primers specific for miRNAs with published implications in stem cell self-renewal, hematopoiesis, neuronal development and differentiated tissue identification and also includes U6 as a normalisation signal.

Stem Cells MicroRNA qPCR Array Layout

1 2 3 4 5 6 7 8 9 10 11 12
A miR-
18a
miR-
19a
miR-
19b
miR-
24
miR-
25
miR-
17-5p
miR-
30c
miR-
34a
miR-
106a
miR-
106b
miR-
130b
miR-
141
B miR-
150
miR-
199a
miR-
200b
miR-
200c
miR-
301
miR-
302a
miR-
302b
miR-
302c
miR-
302d
miR-
367
miR-
368
miR-
369-5p
C miR-
369-3p
miR-
370
miR-
371
miR-
372
miR-
373
let-
7a
let-
7b
miR-
10a
miR-
10b
miR-
16
miR-
17-3p
miR-
20a
D miR-
20b
miR-
23a
miR-
23b
miR-
26a
miR-
26b
miR-
30b
miR-
30d
miR-
32
miR-
33
miR-
92
miR-
93
miR-
99a
E miR-
101
miR-
107
miR-
126
miR-
130a
miR-
142-5p
miR-
142-3p
miR-
146a
miR-
146b
miR-
155
miR-
181a
miR-
181b
miR-
181c
F miR-
181d
miR-
191
miR-
193a
miR-
193b
miR-
197
miR-
221
miR-
223
miR-
339
miR-
9
miR-
103
miR-
124a
miR-
125a
G miR-
125b
miR-
127
miR-
128a
miR-
128b
miR-
132
miR-
134
miR-
135a
miR-
135b
miR-
136
miR-
138
miR-
149
miR-
153
H miR-
154
miR-
183
miR-
218
miR-
219
miR-
222
miR-
1
miR-
122a
miR-
133a
miR-
133b
miR-
195
miR-
206
U6
snRNA
Stem Cell Signature Set
Hematopoietic Set
Neuronal Set
Organ Set

QuantiMir™ Kits
The stem cell microRNA qPCR array can be used in conjunction with the QuantiMir™ RT kit to create a small RNA quantification system. This system rapidly tags and converts all small RNAs into detectable uniform cDNA for qPCR using a simple and robust procedure. This technology can convert enough RNA to cDNA for up to 5,000 qPCR measurements making it ideal for validating newly discovered microRNAs and detect and quantitating siRNAs in knock-down experiments.

Material Available for Download
Stem Cell MicroRNA qPCR Array User Manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Stem Cell MicroRNA Profiling Panel RA620A-1 · System Biosciences
RA620A-1
System Biosciences
10 profiles £999.00
10 profiles
view
QuantiMir Kit RA420A-1 · System Biosciences
RA420A-1
System Biosciences
20 reactions £535.00
20 reactions
view