Cambridge Bioscience offers a stem cell microRNA qPCR panel and the QuantiMir™ to simultaneously measure the expression of 95 miRNAs associated with stem cell renewal, haematopoiesis, neural development and tissue patterning down to picogram quantities in total RNA samples.
Benefits of Using the Stem Cell qPCR Array & QuantiMir™ System
• Simultaneously profile 95 different MicroRNAs known to be involved in stem cell self-renewal, haematopoiesis, neural development and tissue patterning
• Identify miRNA development biomarkers and expression pattern signatures in Stem Cells
• System rapidly tags and converts all small RNAs into detectable cDNA for qPCR
• Simple and robust procedure
• Sensitive and quantitative down to picogram-quantities of starting total RNA
• Compatible with high-throughput screening of numerous cell lines and tissues
Stem Cell MicroRNA qPCR Array
Supplied in a convenient 96-well plate format, segmented into 4 separate stem cell pathways, this array contains 95 primers specific for miRNAs with published implications in stem cell self-renewal, hematopoiesis, neuronal development and differentiated tissue identification and also includes U6 as a normalisation signal.
Stem Cells MicroRNA qPCR Array Layout
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | miR- 18a |
miR- 19a |
miR- 19b |
miR- 24 |
miR- 25 |
miR- 17-5p |
miR- 30c |
miR- 34a |
miR- 106a |
miR- 106b |
miR- 130b |
miR- 141 |
| B | miR- 150 |
miR- 199a |
miR- 200b |
miR- 200c |
miR- 301 |
miR- 302a |
miR- 302b |
miR- 302c |
miR- 302d |
miR- 367 |
miR- 368 |
miR- 369-5p |
| C | miR- 369-3p |
miR- 370 |
miR- 371 |
miR- 372 |
miR- 373 |
let- 7a |
let- 7b |
miR- 10a |
miR- 10b |
miR- 16 |
miR- 17-3p |
miR- 20a |
| D | miR- 20b |
miR- 23a |
miR- 23b |
miR- 26a |
miR- 26b |
miR- 30b |
miR- 30d |
miR- 32 |
miR- 33 |
miR- 92 |
miR- 93 |
miR- 99a |
| E | miR- 101 |
miR- 107 |
miR- 126 |
miR- 130a |
miR- 142-5p |
miR- 142-3p |
miR- 146a |
miR- 146b |
miR- 155 |
miR- 181a |
miR- 181b |
miR- 181c |
| F | miR- 181d |
miR- 191 |
miR- 193a |
miR- 193b |
miR- 197 |
miR- 221 |
miR- 223 |
miR- 339 |
miR- 9 |
miR- 103 |
miR- 124a |
miR- 125a |
| G | miR- 125b |
miR- 127 |
miR- 128a |
miR- 128b |
miR- 132 |
miR- 134 |
miR- 135a |
miR- 135b |
miR- 136 |
miR- 138 |
miR- 149 |
miR- 153 |
| H | miR- 154 |
miR- 183 |
miR- 218 |
miR- 219 |
miR- 222 |
miR- 1 |
miR- 122a |
miR- 133a |
miR- 133b |
miR- 195 |
miR- 206 |
U6 snRNA |
| Stem Cell Signature Set | |
| Hematopoietic Set | |
| Neuronal Set | |
| Organ Set |
QuantiMir™ Kits
The stem cell microRNA qPCR array can be used in conjunction with the QuantiMir™ RT kit to create a small RNA quantification system. This system rapidly tags and converts all small RNAs into detectable uniform cDNA for qPCR using a simple and robust procedure. This technology can convert enough RNA to cDNA for up to 5,000 qPCR measurements making it ideal for validating newly discovered microRNAs and detect and quantitating siRNAs in knock-down experiments.
Material Available for Download
Stem Cell MicroRNA qPCR Array User Manual
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| Stem Cell MicroRNA Profiling Panel RA620A-1 · System Biosciences | RA620A-1 System Biosciences |
10 profiles |
£999.00
10 profiles
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|
| QuantiMir Kit RA420A-1 · System Biosciences | RA420A-1 System Biosciences |
20 reactions |
£535.00
20 reactions
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