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Apoptotic & Necrotic Cell Quantification Kits

Biotium offers fast and sensitive assays for the simultaneous quantification of apoptotic and necrotic cells within the same cell population by flow cytometry or fluorescence microscopy. Using either Annexin V labelled with fluorescein (FITC) or Annexin V labelled with CF™488A (Plus Kit) and the ethidium homodimer III, apoptotic and necrotic cells are identified by highlighting them with green and red fluorescence respectively.

Benefits of using Apoptotic and Necrotic Cell Quantification Kits
• Enables simultaneous detection of apoptotic and necrotic cells in a single assay
• Simple to use
• Single 15-30 minute staining step
• EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells
• Analysis by flow cytometry or fluorescence microscopy

1. Detecting apoptotic cells - green
Apoptotic and Necrotic Cell Quantification Kit - BT30017
During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. Using Annexin V, with a high affinity for PS, labelled with fluorescein (FITC) (2+λabs/λem= 492/ 514 nm) this kit identifies apoptotic cells by highlighting them with green fluorescence by binding to PS exposed on the outer leaflet.

Apoptotic and Necrotic Cell Quantification Kit - BT30065
During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. Using Annexin V labelled with CF™488A (Abs/Em 490/515 nm), this kit identifies apoptotic cells by highlighting them with green fluorescence by binding to PS exposed on the outer leaflet. CF™488A is spectrally similar to fluorescein (FITC), with a much brighter and more photostable fluorescence.

2. Detecting necrotic cells - red
During necrosis, both internal organelle and plasma membrane integrity are lost, resulting in spilling of cytosolic and organellar contents into the surrounding environment. Utilising Ethidium homodimer III (EtD-III), a highly positively charged nucleic acid probe which is impermeant to live cells or apoptotic cells, this kit stains and identifies necrotic cells with red fluorescence (λabs/λem = 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I used in other kits due to its significantly higher affinity for DNA and higher fluorescence quantum yield.

Material available for download
Apoptosis and Necrosis Quantification Kit (BT30017) protocol
Apoptosis and Necrosis Quantitation Kit Plus (BT30065) protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Apoptosis & Necrosis Quantification Kit (50 assays) BT30017 · Biotium
BT30017
Biotium
1 kit £203.00
1 kit
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Apoptosis and Necrosis Quantitation Kit Plus (50 assays) BT30065 · Biotium
BT30065
Biotium
1 kit £196.00
1 kit
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