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NGS library preparation kits for limited samples

The Accel-NGS 1S DNA library kits from Swift Biosciences allow next-generation sequencing (NGS) library construction from samples with limiting amounts of DNA including damaged, denatured and single stranded DNA. Featuring Swift's Adaptase® technology, these kits allow you to capture previously unattainable sequences using your platform of choice, while preserving input fragmentation patterns for the precise mapping of DNA insert ends.

Benefits of using the Accel-NGS 1S DNA library kits
• Supports inputs as low as 10 pg
• Simple, 2-hour protocol
• High fidelity polymerase
• Adapts DNA > 40 bp long
• Minimises sequence bias from PCR
• Enables the capture of previously unattainable sequences

Sample types
• Single-stranded DNA (ssDNA) including first-strand cDNA
• Damaged (nicked) DNA
• Samples of mixed ssDNA & double-stranded DNA (dsDNA)
• Heat-denatured pathogenic samples
• Single-stranded genomes (viruses, phage) & oligonucleotides
• Formalin-fixed paraffin-embedded (FFPE)
• Ancient DNA

Swift Adaptase technology
Swift’s Adaptase technology allows for minimal adapter dimers with no need for adapter titration. Adaptase is single strand compatible, and so maximises recovery of DNA from bisulfite-converted samples, or other damaged and fragmented DNA or RNA samples.

Accel-NGS 1S DNA library kits workflow

Accel-NGS 1S DNA Library Kits workflow

The simple 2 hour protocol requires 10 pg - 250 ng input DNA

Applications
• Whole genome sequencing
• Microbial sequencing
• Metagenomics
• ChIP sequencing
• RNA sequencing

Formats available
Accel-NGS® 1S Plus DNA library kit for Illumina®
Accel-NGS® DNA library kit for the Ion Torrent™ platform

Material available for download
Accel-NGS® 1S Plus DNA library kit product sheet
Accel-NGS® 1S Plus DNA library kit protocol
Accel-NGS® DNA library kit for the Ion Torrent™ platform protocol
ASM 2016 poster - Complete microbial community representation by next-generation sequencing

Accel-NGS 1S DNA library kit citations

Ion Torrent™ is a trademark of Thermo Fisher Scientific.
Illumina® is a registered trademark of Illumina, Inc.

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