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Episomal DNA Vectors: Minicircles

The Minicircle Technology from System Biosciences (SBI) is is a great option for gene expression. Produced as small, excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences and comes with a variety of promoter and reporter combinations. Featuring a smaller size, Minicircles facilitate more efficient transfection than standard-sized plasmids, and although they do not replicate in the host cell, expression lasts for 14 days or longer in dividing cells and can continue for months in non-dividing cells.

Benefits of using Minicircle technology
• Episomal expression sustained over weeks
• Foreign DNA-free
• Unlimited insert size
• Optimised coli minicircle production strain
• Effective in vitro and in vivo
• Choice of promoter, reporter gene and vector format

minicircle technology

Generating minicircle DNA from the parental minicircle plasmid

Generating minicircle DNA from the parental minicircle plasmid

Easy, sustained transfection in most cell types. Transfection of 1 μg of minicircle DNA (pMC.CMV-MCS-EF1-GFPSV40PolyA) into HEK293 cells delivers over one week of robust gene expression.

Please note: Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle DNA production to both not-for-profit and commercial end users. Please contact our SBI specialists for additional details.

Material available for download
MC-Easy™ Minicircle Production Kit brochure

Minicircle citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Minicircle Single Reporter: CMV-Luciferase Parental Plasmid (10 µg) BLIV510MN-1 · System Biosciences
BLIV510MN-1
System Biosciences
10 µg £974.00
10 µg
view
Minicircle Single Reporter: EF1 Luciferase DNA (30 µg) BLIV511MC-1 · System Biosciences
BLIV511MC-1
System Biosciences
30 µg £705.00
30 µg
view
Minicircle Single Reporter: EF1 alpha-Luciferase Parental Plasmid (10 µg) BLIV511MN-1 · System Biosciences
BLIV511MN-1
System Biosciences
10 µg £974.00
10 µg
view