High complexity NGS library preparation

The Accel-NGS 2S DNA library kits from Swift Biosciences use proprietary technology to generate libraries with low duplication rates and high complexity, even from highly damaged FFPE or low input circulating cell-free DNA (cfDNA) samples, supporting double-stranded DNA (dsDNA) inputs as low as 10 pg. Including kits to support a range of whole genome sequencing (WGS) applications and products designed to minimise duplicates or provide PCR-free next-generation sequencing (NGS) preparation, the Accel-NGS 2S product family benefits from specific protocol recommendations for various oncology-related sample types.

Benefits of using the Accel-NGS 2S DNA library kits
• Ideal for difficult samples
• Input as low as 10 pg DNA
• Single-tube processing
• Simple protocol
• Compatible with Illumina® platforms
• Automation compatible for high throughput

Sample types
• Double stranded DNA (dsDNA)
• Damaged FFPE samples
• Cell free DNA (cfDNA)
• ChIP-Seq

Included within this product range, the Accel-NGS 2S Plus DNA Library Kit supports inputs as low as 10 pg and offers exceptional performance for whole genome sequencing of limiting samples such as FFPE and ChIP-Seq.

Benefits of using the Accel-NGS 2S Plus DNA Library Kit
• Exceptional yields from DNA inputs of 10 pg to 250 ng
• Sequential repair steps
• High complexity libraries for deep sequencing
• No base composition bias from AT-/GC-rich genomes
• No adapter titration or heat steps involved
• “With bead” workflow
• Readily automatable

Accel-NGS 2S DNA kits

*PCR-free capability is determined by sample quality and input quantity. Accel-NGS 2S PCR-free libraries can be generated from as low as 100 ng of high quality
genomic DNA or 10 ng of circulating, cfDNA.

Formats available
Accel-NGS 1S PCR-Free DNA library kit
Accel-NGS 2S Plus DNA library kit

Material available for download
Accel-DNA 2S DNA library kits datasheet
Accel-NGS 2S Plus DNA library kit (Single Indexing for Set A, B Indexing Kit & Set S1-S4 Indexed Adapter)
Accel-NGS 2S Plus DNA library kit (Single Indexing and MIDs: Set A, B Indexing Kit & Set S1-S4 Indexed Adapter)
Accel-NGS 2S Plus DNA library kit (Dual Indexing for Cat. No. 28096)
Accel-NGS 2S Plus with UDIs
Accel-NGS 2S Set A and B Indexing Kit Master Mix volume calculator
Accel-NGS 2S Set S1-S4 Indexed Adapter Master Mix volume calculator
Application note: approaching single-cell sequencing by understanding NGS library complexity & bias

Accel-NGS 2S DNA library kits citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Accel-NGS 2S Plus DNA Library Kit for Illumina 21024 · Swift Biosciences
21024
Swift Biosciences
24 reactions £696.00
24 reactions
view
Accel-NGS 2S PCR-Free Library Kit for Illumina 20024 · Swift Biosciences
20024
Swift Biosciences
24 reactions £649.00
24 reactions
view
2S Set A+B MID Indexing Kit - (Sets A & B) 27396 · Swift Biosciences
27396
Swift Biosciences
96 reactions £788.00
96 reactions
view
2S Set B MID Indexing Kit - (Set B) 27248 · Swift Biosciences
27248
Swift Biosciences
48 reactions £394.00
48 reactions
view
2S Set A MID Indexing Kit - (Set A) 27148 · Swift Biosciences
27148
Swift Biosciences
48 reactions £394.00
48 reactions
view
2S Dual Indexing Kit for Illumina 28096 · Swift Biosciences
28096
Swift Biosciences
96 reactions £510.00
96 reactions
view
2S Indexing Kit for Illumina - ( Sets A & B) 26396 · Swift Biosciences
26396
Swift Biosciences
96 reactions £510.00
96 reactions
view
2S Indexing Kit for Illumina - (Set B) 26248 · Swift Biosciences
26248
Swift Biosciences
48 reactions £255.00
48 reactions
view
2S Indexing Kit for Illumina - (Set A) 26148 · Swift Biosciences
26148
Swift Biosciences
48 reactions £255.00
48 reactions
view
Accel-NGS 2S Plus DNA Library Kit for Illumina 21096 · Swift Biosciences
21096
Swift Biosciences
96 reactions £2596.00
96 reactions
view
Accel-NGS 2S PCR-Free Library Kit for Illumina 20096 · Swift Biosciences
20096
Swift Biosciences
96 reactions £2410.00
96 reactions
view