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Exceptional Cloning With Transcription & Translation-Free Vectors
Stellaris® RNA FISH Technology
Fixable & Non-Fixable Neuronal Terminal Dyes

Transfection Ready Plasmid DNA In 20 Minutes With ZymoPURE II

Stellaris® Positive Control Probe Set

Stellaris® Positive Control Probe Set

Stellaris® ShipReady control probe sets for Stellaris® RNA FISH provide convenient, ready-to-ship positive controls against reference messenger RNAs and long non-coding RNAs (mRNAs and lncRNAs). Validated on cultured cells or whole mount tissues, both qualitative (localisation) and quantitative (transcript counting) controls are available in the most commonly used model organisms including Human, Mouse, C. elegans, Drosophila and Yeast.  Stellaris® ShipReady control probe sets are supplied as 1 nmol of pooled oligos which is sufficient for 40-80 hybridisations.

Stellaris® RNA FISH Technology
Offering an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. Both highly sensitive and specific, a Stellaris® RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts.

How The Stellaris® FISH Technology Works
When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. The inherent redundancies of this direct detection method minimize the possibility of false negative and false positive signals. Positive signals are identified from combined localised fluorescence of multiple probes. Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation.

Benefits Of Using The Stellaris® FISH Technology
• Simple protocol
• Inexpensive
• Platform-independent
• Same-day results
• Compatible with many sample types & applications
• Multiplex RNA target detection
• No isolation, purification or amplification of RNA required

Stellaris® FISH Technology Portfolio - Find out more about the full range


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