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ZymoBIOMICS Microbial Community Standards
Microbiomic & Metagenomic Research Tools
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Optimise Your Microbiomics Workflows with ZymoBIOMICS™ Microbial Community Standards

ZymoBIOMICS™ spike-in control I

ZymoBIOMICS™ spike-in control I

The ZymoBIOMICS™ spike-in control I from Zymo Research offers an in situ positive control for absolute quantification of cell numbers using DNA-sequencing based microbiome measurements. Comprised of equal numbers of two bacteria strains (Imtechella halotolerans (Gram-positive) and Allobacillus halotolerans (Gram-negative)) alien to the human microbiome, the ZymoBIOMICS™ spike-in control I can expose potential bias during DNA extraction.

Benefits of using ZymoBIOMICS™ Spike-in Control I (High Microbial Load)
• Enables absolute cell number quantification with NGS
• In situ quality control for accurate quantification
• Unique composition of bateria alien to the human microbiome

ZymoBIOMICS™ Spike-in Control I workflow

ZymoBIOMICS™ Spike-in Control I Workflow

1. Add ZymoBIOMICS™ Spike-in Control I to sample
2. DNA extraction, library Preparation & Next-Generation Sequencing
3. Bioinformatic analysis to measure absolute microbial abundance

Defined composition

Spike-In Species
Per Prep (20 µl)
 
Cells
16S Copies
Total DNA (ng)
Imtechella halotolerans
2 x 107
6.0 x 107
67.2
Allobacillus halotolerans
2 x 107
1.4 x 108
58.2

Quantify absolute abundance of microbes

Quantify absolute abundance of microbes - ZymoBIOMICS™ Spike-in Control I (High Microbial Load)

The ZymoBIOMICS® Spike-in Control I was added to a fecal sample. Total DNA was then extracted using the ZymoBIOMICS® DNA Miniprep Kit, a library was prepared and then analyzed by 16S targeted Next-Gen Sequencing. From the resulting data, the percentage of Imtechella halotolerans and Allobacillus halotolerans in the total sample composition was then calculated. The resulting percentage was paired to the defined Spike-in I cell input and used to calculate the absolute abundance for organisms in the sample based their sample composition percentages.

Material available for download
ZymoBIOMICS™ Spike-in Control I (High Microbial Load) protocol

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