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Complex siRNA Pools For Gene Silencing

Highly Efficient Ribosomal Depletion In Any Species
DNA/RNA Shield™ Microbial Pathogen Sample Collection Tubes

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

riboPOOLs for ribosomal RNA depletion

riboPOOLs for ribosomal RNA depletion

riboPOOLs are an affordable and flexible solution for ribosomal RNA (rRNA) depletion or other custom RNAs from any species. Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs allow the efficient removal of abundant rRNAs from total RNA samples isolated from prokaryotic or eukaryotic cells or tissues. 

Benefits of using riboPOOL
• Highly efficient and specific
• Any species
• Low cost solution
• Deplete custom RNA
• Applicable for nonpolyadenylated RNA
• Custom scales available

riboPOOL workflow

riboPOOL Workflow

riboPOOL technology
riboPOOLs consist of complex mixtures of single-stranded 3’biotinylated oligonucleotide-probes designed to specifically hybridise with all ribosomal RNAs of the target species. Ribosomal RNAs bound to riboPOOL-probes are efficiently removed from the samples by streptavidin-conjugated magnetic beads. riboPOOLs work independent of RNA polyadenylation and can be applied for any downstream application e.g. NGS library preparation.

riboPOOL formats

riboPOOL

In Silico validated

Experimentally validated

Human
ü
ü
Mouse/rat
ü
ü Mouse
Planarian
ü
ü
Pan-prokaryote (designed against 76 representative species from all common bacteria/archaebacterial phyla)
ü
ü (S.aureus, E.coli, Salmonella, Helicobacter, Fusobacterium, Campylobacter, Vibrio, C.difficile)
Yeast (S.cerevisiae)
ü
 
Drosophila
ü
 
Plant (Arabidopsis Thaliana)
ü
 
Globin (mRNA)
ü
 
E.coli
ü
 
S.Aureus
ü
 
B.Subtilis
ü
 
P.Aeruginosa
ü
 
S.Enterica
ü
 
Stenotrophomonas sp.
ü
 

*Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species

Material available for download
riboPOOL brochure
Pan-prokaryote riboPOOL flyer
riboPOOL protocol

Ribo-Zero is a trademark of Illumina, Inc

Products

Note: availability depends on country - see product detail page.

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Contact Our siTOOLs Biotech Specialist, Doaa

siTOOLs Biotech

Request a free sample of the pan-prokaryote riboPOOL

Request to receive a free sample of the pan-prokaryote riboPOOL. Strictly while stocks last.