riboPOOLs for ribosomal RNA depletion

riboPOOLs are an affordable and flexible solution for ribosomal RNA (rRNA) depletion or other custom RNAs from any species from siTOOLs. Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs allow the efficient removal of abundant rRNAs from total RNA samples isolated from prokaryotic or eukaryotic cells or tissues. 

Benefits of using riboPOOL
• Highly efficient and specific
• Any species
• Low cost solution
• Deplete custom RNA
• Applicable for nonpolyadenylated RNA
• Custom scales available

riboPOOLs can be provided as either:
• Probes alone with nuclease-free water. Please refer to the protocol for reagents required.
• Complete riboPOOL kits that include buffer, steptavidin-magnetic beads & ethanol precipitation reagents.

riboPOOL workflow

riboPOOL workflow

riboPOOL technology
riboPOOLs consist of complex mixtures of single-stranded 3’biotinylated oligonucleotide-probes designed to specifically hybridise with all ribosomal RNAs of the target species. Ribosomal RNAs bound to riboPOOL-probes are efficiently removed from the samples by streptavidin-conjugated magnetic beads. riboPOOLs work independent of RNA polyadenylation and can be applied for any downstream application e.g. NGS library preparation.

riboPOOL formats
Choose from a diverse list of ready-made riboPOOLs or custom-design a riboPOOL for any species or RNA of choice.

riboPOOLs for eukaryotes riboPOOLs for prokaryotes
Homo sapien (human) Pan-Prokaryote (Universal Microbe)
Mus musculus/Rattus norvegicus (mouse/rat) Pan-Prokaryote v2 (Universal Microbe)
Drosophila melanogaster (fruitfly) Pan-Bacteria
Arabidopsis thaliana (plant) Pan-Archaea
Danio rerio (zebrafish) Escherichia coli
Sacchromyces cerevisiae (yeast) Pseudomonas aeruginosa
Pichia pastoris (yeast) Staphylococcus aureus
Bombyx morii (silkworm) Bacillus subtilis
Ustilago maydis (fungus) Salmonella enterica
Amphimedon queenslandica (sponge) Stenotrophomonas sp.
Schmidtea mediterranea (planaria) Caulobacter crescentus
Plautia stali (brown winged green bug) Haloferax volcanii
Chinchilla Laginera (rodent)  
Oryza sativa (rice) riboPOOLs for abundant RNAs
Loripes orbiculatus + Lucinoma aequizonata (clams) Human Globin (mRNA)


riboPOOLs may be combined in custom ratios to form a combination riboPOOL that can be used for samples across multiple species

Material available for download
riboPOOL protocol
riboPOOL brochure
Pan-prokaryote riboPOOL flyer
New pan-prokaryote riboPOOLs flyer
riboPOOL application note - efficient ribosomal RNA depletion across multiple species & input amounts in 70 mins

riboPOOL citations

Ribo-Zero is a trademark of Illumina, Inc

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
riboPOOL 2 rdS02001 · siTOOLs Biotech
rdS02001
siTOOLs Biotech
2 nmol £377.00
2 nmol
view
riboPOOL 5 rdS05001 · siTOOLs Biotech
rdS05001
siTOOLs Biotech
5 nmol £781.00
5 nmol
view
riboPOOL 10 rdS10001 · siTOOLs Biotech
rdS10001
siTOOLs Biotech
10 nmol £1337.00
10 nmol
view
Combination riboPOOL 2 rdS02002 · siTOOLs Biotech
rdS02002
siTOOLs Biotech
2 nmol £573.00
2 nmol
view
Combination riboPOOL 5 rdS05002 · siTOOLs Biotech
rdS05002
siTOOLs Biotech
5 nmol £1273.00
5 nmol
view
Combination riboPOOL 10 rdS10002 · siTOOLs Biotech
rdS10002
siTOOLs Biotech
10 nmol £2147.00
10 nmol
view
Combination riboPOOL kit (up to 4 riboPOOLs) rdSKC1201 · siTOOLs Biotech
rdSKC1201
siTOOLs Biotech
12 reactions £1032.00
12 reactions
view
Combination riboPOOL kit (up to 4 riboPOOLs) rdSKC2401 · siTOOLs Biotech
rdSKC2401
siTOOLs Biotech
24 reactions £1905.00
24 reactions
view
riboPOOL kit (human/mouse) rdSKH2401 · siTOOLs Biotech
rdSKH2401
siTOOLs Biotech
24 reactions £1270.00
24 reactions
view
riboPOOL kit (other species/Pan-prokaryote/Globin) rdSKO1201 · siTOOLs Biotech
rdSKO1201
siTOOLs Biotech
12 reactions £873.00
12 reactions
view
riboPOOL kit (human/mouse) rdSKH9601 · siTOOLs Biotech
rdSKH9601
siTOOLs Biotech
96 reactions £4317.00
96 reactions
view
Combination riboPOOL kit (up to 4 riboPOOLs) rdSKC9601 · siTOOLs Biotech
rdSKC9601
siTOOLs Biotech
96 reactions £6348.00
96 reactions
view
riboPOOL kit (other species/Pan-prokaryote/Globin) rdSKO2401 · siTOOLs Biotech
rdSKO2401
siTOOLs Biotech
24 reactions £1587.00
24 reactions
view
riboPOOL kit (other species/Pan-prokaryote/Globin) rdSKO9601 · siTOOLs Biotech
rdSKO9601
siTOOLs Biotech
96 reactions £5332.00
96 reactions
view
riboPOOL kit (human/mouse) rdSKH1201 · siTOOLs Biotech
rdSKH1201
siTOOLs Biotech
12 reactions £715.00
12 reactions
view

Request a free sample of the pan-prokaryote riboPOOL

Request to receive a free sample of the pan-prokaryote riboPOOL. Strictly while stocks last.