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Microbiomic & Metagenomic Research Tools
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Microbial DNA Isolation From Host Nucleic Acids - HostZERO™

Microbial DNA Isolation From Host Nucleic Acids - HostZERO™

Cambridge Bioscience offers a kit to effectively isolate microbial DNA from contaminating host nucleic acid in microbial samples. Combining new technology with the ZymoBIOMICS® non-biased purification system, the HostZERO™ Kit does not introduce bias or cause sample loss.

HostZERO™ Microbial DNA Kit Workflow

HostZERO™ Microbial DNA Kit Workflow

Benefits Of Using The HostZERO™ Microbial DNA Kit
• Simple host depletion & microbial isolation process
• Increases the ratio of bacterial to host DNA
• Maintains accurate microbial sample profile
• Retains high amounts of bacteria DNA

HostZERO™ Microbial DNA Isolation Kit Depletes Greatest Amount Of Human DNA From A Microbial Sample

The HostZERO™ Microbial DNA Isolation Kit depletes the greatest amount of human DNA from a microbial sample.

Real-time PCR was used to evaluate eluates recovered from aliquots of a human saliva sample using the HostZERO™ Kit & kits from Suppliers Q & M. The composition of the purified DNA is shown in terms of bacterial and human DNA abundance. The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Bacterial DNA Is Effectively Recovered From Human Saliva Sample with HostZERO™ Technology

High Recovery of Microbial DNA with HostZERO

Real-time PCR was used to evaluate the yield of bacterial DNA in eluates recovered from aliquots of a human saliva sample using the HostZERO™ Kit and kits from Suppliers Q and M. The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Composition Of The Microbial Sample Is Maintained Best In Samples Treated With HostZERO™

Non-biased Process Using HostZERO

The cDNA recovered from aliquots of a human saliva sample using the HostZERO™ Kit and kits from Suppliers Q and M was processed with 16S rRNA gene targeted sequencing. Primers targeting the 16S V3-V4 region were used, and the amplicons were sequenced on the Illumina MiSeq® (2x300bp). The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Applications
• Microbiomics
• Pathogen research
• Bacterial disease research

Material Available For Download
HostZERO™ Microbial DNA Brochure
HostZERO™ Microbial DNA Protocol

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