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Microbiomic & Metagenomic Research Tools
ZymoBIOMICS Microbial Community Standards
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Microbial DNA isolation from host nucleic acids - HostZERO™

Microbial DNA isolation from host nucleic acids - HostZERO™

Zymo Research's HostZERO™ kit effectively isolates microbial DNA from contaminating host nucleic acid in microbial samples. Combining new technology with the ZymoBIOMICS® non-biased purification system, the HostZERO™ kit does not introduce bias or cause sample loss.

HostZERO™ microbial DNA kit workflow

HostZERO™ Microbial DNA Kit Workflow

Benefits of using the HostZERO™ microbial DNA kit
• Simple host depletion and microbial isolation process
• Increases the ratio of bacterial to host DNA
• Maintains accurate microbial sample profile
• Retains high amounts of bacteria DNA

HostZERO™ microbial DNA isolation kit depletes greatest amount of human DNA from a microbial sample

The HostZERO™ Microbial DNA Isolation Kit depletes the greatest amount of human DNA from a microbial sample.

Real-time PCR was used to evaluate eluates recovered from aliquots of a human saliva sample using the HostZERO™ Kit and kits from Suppliers Q and M. The composition of the purified DNA is shown in terms of bacterial and human DNA abundance. The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Bacterial DNA is effectively recovered from human saliva sample with HostZERO™ technology

High Recovery of Microbial DNA with HostZERO

Real-time PCR was used to evaluate the yield of bacterial DNA in eluates recovered from aliquots of a human saliva sample using the HostZERO™ Kit and kits from Suppliers Q and M. The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Composition of the microbial sample is maintained best in samples treated with HostZERO™

Non-biased Process Using HostZERO

The cDNA recovered from aliquots of a human saliva sample using the HostZERO™ Kit and kits from Suppliers Q and M was processed with 16S rRNA gene targeted sequencing. Primers targeting the 16S V3-V4 region were used, and the amplicons were sequenced on the Illumina MiSeq® (2x300bp). The control method is the ZymoBIOMICS® DNA Microprep Kit, which extracts total DNA from the sample without host DNA depletion.

Applications
• Microbiomics
• Pathogen research
• Bacterial disease research

Material available for download
HostZERO™ Microbial DNA Brochure
HostZERO™ Microbial DNA Protocol

Products

Note: availability depends on country - see product detail page.

Speak To Our Zymo Research Specialist Emma