LoopSeq Transcriptome kit

LoopSeq™ Transcriptome library preparation kits enable the generation of long read data from short read Illumina® sequencers with no extra hardware required. Using a set of enzymes to propagate a barcode intramolecularly through any piece of DNA or RNA, the LoopSeq kits use a simple, single tube workflow to eliminate PCR and GC bias as molecules are labelled in the first step and only sequenced/reported once. With significantly reduced error rates compared to those of PacBio® and Oxford Nanopore®, LoopSeq Transcriptome kits accurately determine not only transcript abundance but also unique isoforms.

Benefits of using these LoopSeq kits
• Simple, single tube workflow 
• Highly accurate long-read sequencing
• Unique molecular identifier (UMI)-based transcript counting – barcode based quantification with no PCR bias

LoopSeq workflow

LoopSeq workflow

1. Get the kit
2. Upload results - securely upload FASTQ results to Loop's cloud pipeline
3. Receive data - download FASTQ and CSV files with classification and quantification data

LoopSeq Workflow

LoopSeq technology

LoopSeq technology

LoopSeq technology uses single-molecule counting, which makes it possible to calculate true transcript abundance using barcode based quantification with no PCR bias.

Comparison of Loop Genomics technology vs. Illumina® technology using an ERCC control sample

Comparison of Loop Genomics technology vs. Illumina® technology using an ERCC control sample.

TruSeq® (left) and LoopSeq Transcriptome kit (right) comparison. TruSeq® provides short reads and quantification via coverage whereas LoopSeq provides synthetic long reads from short reads with single-molecule quantification. Data shows that the LoopSeq Transcriptome kit provides a much more accurate quantification of the synthetic ERCC synthetic control sample.

Product information

Assay time 8.5 hours
Hands-on time 2.5 hours
Mechanism of action Synthetic long-read sequencing
Multiplexing Allows for 8 samples to be processed in one tube
Input quantity 10 ng of total RNA
Category Transcriptome
System compatibility HiSeq® 2500, HiSeq® 3000, HiSeq® 4000, NextSeq®, NovaSeq®, MiSeq®, MiniSeq®
Nucleic acid type RNA
Method 2 x 150 Paired End (PE) sequencing
Specialised sample types Eukaryotic sample
Technology Assembly of synthetic long-reads from short-reads
Automation capability Yes


Applications
• Full length single molecule isoform discovery and examination of splicing
• Detection of structural variants (segmental duplications, gene loss and fusion events)
• Detect RNA editing SNPs
• UMI-based transcript counting

Material available for download
LoopSeq Transcriptome kit protocol
LoopSeq Transcriptome kit quick start guide

Illumina, HiSeq, NextSeq, MiSeq, NovaSeq, MiniSeq and TruSeq are registered trademarks of Illumina, Inc. Oxford Nanopore is a trademark of Oxford Nanopore Technologies. Pacific Biosciences is a registered trademark of Pacific Biosciences.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
LoopSeq™ Transcriptome Kit LGCRNA8X3RXN · Loop Genomics
LGCRNA8X3RXN
Loop Genomics
24 reactions £1730.00
24 reactions
view