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Long-Read Transcriptome Seq From Illumina® Sequencers - LoopSeq™

Long-Read Transcriptome Seq From Illumina® Sequencers - LoopSeq™

Cambridge Bioscience offers transcriptome library preparation kits enabling the generation of long read data from short read Illumina™ sequencers with no extra hardware required. Using a set of enzymes to propagate a barcode intra-molecularly through any piece of DNA or RNA, the LoopSeq™ kits use a simple, single tube workflow to eliminate PCR & GC bias as molecules are labelled in the first step and only sequenced/reported once.

Benefits Of Using These LoopSeq™ Kits
• Single tube workflow after 20 minute labelling step
• 24 individual mRNA samples multiplexed in a single tube
• Full length trancripts
• UMI Based transcriptome counting
• Consensus error correction lowers Illumina output error

LoopSeq™ Workflow

 

​LoopSeq™ Service

1. Get the kit
2. Upload Results - Securely upload FASTQ results to Loop's cloud pipeline
3. Receive Data - download FASTQ and CSV files with classification & quantification data

LoopSeq™ Workflow

Applications
• Full length single molecule isoform discovery & examination of splicing
• Detection of structural variants (segmental duplications, gene loss & fusion events)
• Detect RNA editing SNPs
• UMI-based transcript counting

Illumina is a registered trademark of Illumina, Inc

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Loop Genomics