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Long Read Microbiome Sequencing From Illumina Sequencers
ZymoBIOMICS Spike-In Control I - Quantify Absolute Microbiome Sample Cell Number
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Long read microbiome seq from Illumina® sequencers - LoopSeq™

Long read microbiome seq from Illumina® sequencers - LoopSeq™

LoopSeq™ microbiome sample preparation kits enable long read data from short read Illumina™ sequencers with no extra hardware required. Using a set of enzymes to propagate a barcode intra-molecularly through any piece of DNA or RNA, the LoopSeq™ kits use a simple, single tube workflow to eliminate PCR and GC bias as molecules are labelled in the first step and only sequenced/reported once. 

Benefits of using these LoopSeq™ kits
• Single tube workflow after 20 minute labelling step
• 24 individual microbiome samples multiplexed in a single reaction tube
• Barcode based molecule quantification avoids PCR and GC bias
• Only 10 ng genomic DNA (2 ng/ul) input required
• Consensus error correction lowers Illumina® output error

Benefits 1

Benefits 2

LoopSeq™ workflow


​LoopSeq™ Service

1. Get the kit
2. Upload results - securely upload FASTQ results to Loop's cloud pipeline
3. Receive data - download FASTQ and CSV files with classification and quantification data

LoopSeq™ Workflow

LoopSeq™ versus short read microbiome classification

Long read versus short read microbiome classification

• Full length 16s and 18s (V1-V9)
• Shot gun metagenomics and microbial genome sequencing
• Custom microbiome markers

Material available for download
LoopSeq™ 16S Microbiome 24-plex Kit and LoopSeq™ 16S and 18S Microbiome 24-plex Kit user manual

Illumina is a registered trademark of Illumina, Inc


Note: availability depends on country - see product detail page.

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Applications: NextGen Sequencing

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