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Specific STING Proteins
Functional Kinase Profile Screening Services
Find Oure More About Proteins For STING Research

Innovative detergents for membrane protein extraction

Innovative detergents for membrane protein extraction

These innovative calixarene surfactants enable the purification of functional membrane proteins. Requiring no folding, mutagenesis, truncation or fusion and offering a purification method that maintains the functional and structural integrity of membrane proteins, these detergents are the ideal solution for the extraction and stabilisation of membrane proteins.

Benefits of using these detergents
• Membrane proteins extracted and stabilised
• Functional and structural integrity maintained
• No refolding, mutagenesis, truncation, or fusion requirements
• Native state of membrane proteins unaffected

Cayman detergents

Simulation of multidrug efflux pump subunit AcrB (E. coli strain K12) (PDB 5YIL) and adenosine A2A receptor (PDB 5WF5) being extracted from a lipid membrane bilayer and held in active conformations using calixarene-based detergents.

Binding of radioligand on target protein, purified in reference detergent with or without CALXGLUK as an additive

CALXGLUK Graph

Purified protein was incubated with radioligand in absence (total, blue bars) or presence (Non Specific Signal, red bars) of an excess of cold ligand. After filtration on GF/C membranes and washing, scintillation agent was added and radioactivity was detected using a Microbeta2. Specific radioactivity (green bars) corresponds to (total signal) – (non-specific signal).

Material available for download
CALXGLUK product information
CALXCHOL product information
CALX8 product information

Products

Note: availability depends on country - see product detail page.

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CAS Number: 1872296-37-1
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CAS Number: 1872296-37-1
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CAS Number: 1872296-37-1
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CAS Number: 2121543-66-4
Compound Purity: ≤85%
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CAS Number: 2121543-66-4
Compound Purity: ≤85%
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CAS Number: 1820596-85-7
Compound Purity: ≥95%
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CAS Number: 1820596-85-7
Compound Purity: ≥95%
View product detail
CAS Number: 1820596-85-7
Compound Purity: ≥95%
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