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Ultrasensitive Visible Blue Nucleic Acid Gel Stain

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Frozen Human Whole Cord Blood Buffy Coat

Frozen Human Whole Cord Blood Buffy Coat

Cambridge Bioscience offers UK-sourced, high purity, frozen human cord blood buffy coat. With 99% of red cells removed, the frozen cord buffy coat contains a concentrated supply of > 130 million to 7 billion total nucleated cells (TNCs) including haematopoietic stem cells. These frozen cord blood buffy coats are the only available samples on the market enabling isolation of various cell fractions together from the same donor.

TNC & CD34 Count
The TNC and CD34+ counts are assessed using anti-CD45 and anti-CD34+ staining by FACS, together with a cell viability dye post-thaw on an aliquot of your cord blood unit. An expected true viable TNC or CD34+ post-thaw count will be provided with each sample and samples will be shipped in dry ice.

Benefits Of Using This Frozen Cord Blood Buffy Coat
• 99% red cells removed using patented TotiCyte technology
• > 130 million to 7 billion total nucleated cells
• UK sourced
• Cell viability measurement of TNCs & CD34+ with viability dye & anti-CD45 and anti-CD34+ staining respectively, using FACS
• Human Tissue Authority (HTA) licensed collection & banking facilities
• Collection & banking conforms & complies with the EU Cells & Tissues Directive
• Samples consented for genetic analysis
• Samples carry consent for full ownership of data with the researcher
• Donors pre-screened for HIV I/II,  HBC, HCV, HTLV-I/II & Syphilis TP
• All processing carried out in facilities to GMP & ISO 9001 standards
• Donors pre-screened with detailed questionnaire before selection
• Custom formats available

TotiCyte Technology
Frozen cord blood buffy coat is isolated using novel, patented and CE marked reagent, TotiCyte, which retains 97% of haemopoietic stem cells post processing and at least 63% viability post-thaw. This results in 2.1 times more stem cells and 3 times more cells (CD34+ cells) recovered post-thaw than the current industry leading processing method. TotiCyte also removes 99% of red blood cells, leaving a concentratrated, clearer buffy coat than existing processing methods.

Post-processing viability TotiCyte comparison

Comparison of post-processing viable CD34+ recovery from Frozen Cord Blood Buffy Coats using TotiCyte, industry leader and low-cost isolating reagents systems

Post-thaw viability TotiCyte comparison

Comparison of post-thaw viable CD34+ recovery from Frozen Cord Blood Buffy Coats using TotiCyte, industry leader and low-cost isolating reagents systems

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Note: availability depends on country - see product detail page.

Contact Our Cells4Life Specialist Vashu

Cells4Life - Fresh & Frozen Human Cord Blood Products

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