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Total RNA Purification From Acid-Guanidinium-Phenol Reagents
Mitochondrial ROS Detection Assay Kit

Detergents for Membrane Protein Purification

Achieve High Yield Mammalian Protein Production In Suspension HEK 293 & CHO Derived Cells

Mitochondrial ROS Detection Assay Kit

Mitochondrial ROS Detection Assay Kit

This kit can be used to measure mitochondrial reactive oxygen species (ROS) directly in living adherent or suspension cells. Targeted primarily to the mitochondrion, the mitochondrial ROS assay kit is better suited to single out ROS from other sources in the cell compared to less location-specific DHE and DCFDA dyes.

Benefits of using this Mitochondrial ROS Detection Assay Kit
• Measure mitochondrial ROS directly in living cells
• Includes antimyin A as a positive control
• Flow cytometry, fluorescence and plate-based methods

Mitochondrial ROS Detection Assay Kit

H9C2 cells were stained with 0.62 µM mitochondrial ROS detection reagent following the adherent cells protocol treated with 3 µM antimycin A (A) or vehicle (B). Images were captured one hour after treatment with antimycin A using Biotek®  Cytation™ 5 Multi-Mode Reader.

Mitochondrial ROS Detection Assay Kit

THP-1 cells were stained with 0.5 µM mitochondrial ROS detection reagent following the suspension cells protocol. Cells were treated with 1 µM antimycin A or vehicle and incubated for one hour before measuring on a flow cytometer.

BioTek® is a registered trademark and Cytation™ is a trademark of BioTek Instruments

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