The Quick-16S™ kit from Zymo Research is the fastest and simplest 16s library prep kit available, requiring only 1.5 hours hands-on time to convert up to 96 DNA samples to a single, ready-to-sequence 16S rRNA library. Utilising real-time, quantitative PCR (qPCR) rather than endpoint PCR, the Quick-16S Library Prep Kit enables direct quantification of PCR products, eliminating the need for additional library quantification analysis such as TapeStation® analysis or gel electrophoresis. This kit also incorporates an enzymatic clean-up between the two PCR steps, saving time and reducing costs compared to lengthy AMPure® bead-based clean-ups.
Benefits of using the Quick-16S NGS Library Prep Kit
• Only 1.5 hours hands-on time needed
• No TapeStation® analysis or AMPure® clean-ups required
• Utilisation of real-time PCR limits chimera formation
• Novel primers increase phylogenetic coverage of bacteria and archaea
• Species-level resolution for human microbiome profiling
The total hands-on time for the Quick-16S NGS Library Prep Kit is shorter than that of common library preparation protocols
Total hands-on time calculations are based on the preparation of 96 DNA samples isolated from human stool.
Best phylogenetic coverage
A: The Quick-16S Primer Set V1-V2 includes coverage of common human-associated microbes, including Bifiobacterium, Propionibacterium and Chlamydia, which are missed in common V1-V2 or V1-V3 primers. B: The Quick-16S Primer Set V3-V4 provides up to 87% coverage for archaea, organisms commonly found in the human gut. However, the common V3-V4 primers provide 0% coverage for archaea.
Minimise PCR chimera formation
The Quick-16S NGS Library Prep Kit minimises PCR chimera formation compared to two common protocols: Human Microbiome Project (HMP) and Earth Microbiome Project (EMP). Equivalent amounts of the same faecal DNA sample were used as input. Chimeric sequences were predicted with Uchime (https://www.drive5.com/uchime).
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Note: product availability depends on country - see product detail page.