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Fastest 16S Library Prep - Quick-16S™ NGS Library Prep Kit
Microbiomic & Metagenomic Research Tools
Long Read Microbiome Sequencing From Illumina Sequencers

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Fast library prep for 16S rRNA sequencing - Quick-16S™

Fast library prep for 16S rRNA sequencing - Quick-16S™

The Quick-16S™ kit dramatically reduces the hands-on time of converting up to 96 DNA samples to a single, ready-to-sequence 16S rRNA libraries. With no need for additional library quantification analysis, such as using TapeStation® or gel electrophoresis, or lengthy AMPure® bead-based clean-ups, the Quick-16S™ NGS library prep kit incorporates an enzymatic clean-up between the two PCR steps, saving time and reducing costs.

Benefits of using the Quick-16S NGS Library Prep Kit
• Only 1.5 hours hands-on time needed
• No TapeStation® analysis or AMPure® clean-ups required
• Contains all necessary reagents
• Less than two percent abundance of PCR chimeric sequences
• Increased phylogenetic coverage of bacteria and archaea with novel primers
• Species-level resolution for human microbiome profiling

Best phylogenetic coverage

Quick-16S™ NGS Library Prep Kit - Best Phylogenetic Coverage

A: The Quick-16S Primer Set V1-V2 includes coverage of common human-associated microbes, including Bifiobacterium, Propionibacterium and Chlamydia, which are missed in common V1-V2 or V1-V3 primers. B: The Quick-16S Primer Set V3-V4 provides up to 87% coverage for archaea, organisms commonly found in the human gut. However, the common V3-V4 primers provide 0% coverage for archaea.

Minimise PCR chimera formation

Quick-16S™ NGS Library Prep Kit - Minimise PCR Chimera Formation

The Quick-16S NGS Library Prep Kit minimises PCR chimera formation compared to two common protocols: Human Microbiome Project (HMP) and Earth Microbiome Project (EMP). Equivalent amounts of the same faecal DNA sample were used as input. Chimeric sequences were predicted with Uchime (https://www.drive5.com/uchime).

 

TAPESTATION trademark by Agilent Technologies, Inc. AMPure® is a trademark of BECKMAN COULTER, INC.

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