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Quick Total RNA Purification Kits

DNA Methylation Library Prep Kit

High Efficiency Electroporation Kits

Upgrading Your Laser Scanner? Consider The Sapphire™

RealSeq® Small RNA Library Preparation Kits

RealSeq® Small RNA Library Preparation Kits

Cambridge Bioscience offers a novel, proprietary method for preparation of small RNA sequencing libraries that minimises bias related to intramolecular RNA folding and adapter dimers. Commonly used sequencing library preparations lead to underestimation of the abundance of most miRNAs by as much as 10,000-fold. This can obscure the presence of some RNAs, including ones that could be useful biomarkers if they were detected accurately. Most bias stems from sequence-dependent variability in the enzymatic ligation reactions that attach the two adapters to the 3’ and 5’ ends of the miRNAs /small RNAs during preparation of sequencing libraries. Using a novel single adapter and circularisation, the RealSeq® Sequencing Kits provide an efficient, gel-free method which detects more miRNAs in total RNA than other miRNA library preparation kits. Most importantly, by reducing the number of irrelevant seq reads, sequencing costs are lowered.

Download The ABRF 2018 Poster
Decreasing miRNA Sequencing Bias Using Single Adapter & Circularisation Approach

Formats
RealSeq®-AC Small RNA Library Kit for Illumina® Sequencing
RealSeq®-Biofluids Small RNA Library Kit

RealSeq Technology Schematic

RealSeq Technology

 

Products

Note: availability depends on country - see product detail page.

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Applications: preparation for Next-generation sequencing of small RNAs
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Applications: preparation for Next-generation sequencing of small RNAs
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Applications: preparation for Next-generation sequencing of small RNAs
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Applications: preparation for Next-generation sequencing of small RNAs
View product detail
Applications: preparation for Next-generation sequencing of small RNAs
View product detail
Applications: preparation for Next-generation sequencing of small RNAs
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