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Transfection-Ready Plasmid DNA in <20 Minutes - ZymopPURE II Midi & Maxiprep Kits
Countess™ Slides
Human AB Serum

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

RealSeq®-Biofluids Small RNA Library Kit

RealSeq®-Biofluids Small RNA Library Kit

Cambridge Bioscience offers a novel, proprietary method for preparation of small RNA sequencing libraries that minimises bias related to intramolecular RNA folding and adapter dimers. Offering an efficient, gel-free method which accurately detects cell-free small RNA and optimised for inputs from as little as 50 μl of biofluid, the RealSeq®-Biofluids small RNA library construction kit is the ideal solution for cell-free small RNA library preparation.

Download The ABRF 2018 Poster
Decreasing miRNA Sequencing Bias Using Single Adapter & Circularisation Approach

Benefits Of Using This Kit
• Eliminates bias-induced over/under represented small RNAs
• Efficient & gel-free workflow
• Cell-free small RNA detected
• Optimised for inputs as little as 50 μl of biofluid
• Plasma, serum and urine sample types included
• Detects more miRNAs in total RNA than other miRNA library preparation kits
• Lower sequencing costs due to the reduced number of irrelevant sequencing reads

RealSeq Technology Schematic

RealSeq Schematic

 

RealSeq-biofluids allows sequencing of significantly more miRNAs

RealSeq-Biofluids

Comparison of miRNAs read frequencies of three different library preparation kits (gel-free protocols) from 200 ul plasma samples. Reads were subsampled to 10 million reads per kit and aligned to a reference that includes all human miRNAs listed in miRBase 21 (Miltenyi Biotec). The number of miRNAs detected at different coverage for each library preparation kit is shown. Especially low copy-number miRNAs are significantly more sequenced when the RealSeq® - biofluids kit was used for library preparation.

 

Libraries prepared with RealSeq®-biofluids yield substantially more miRNAs compared to competitor kits

RealSeq Biofluids Library

Sequencing results obtained from commercially available library preparation kits were compared using 200-ul aliquots from the same plasma samples. Short reads are classified as reads <15 nt length after adapter trimming.  Reads passing this filter are then aligned to a reference file with all human miRNAs (miRBase 21, orange), and the remaining reads are aligned to the human genome (hg19, red). RealSeq® - biofluids yields significantly fewer short or unmappable reads (grey) than the other two commercial libraries available that are indicated for biofluids analysis. The number of sequenced miRNAs and of other mappable small RNAs is substantially higher.

Materials Available to Download
RealSeq®-Biofluids Brochure

SomaGenics recommends RNA extraction with Quick-cfRNA columns from Zymo Research.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by SomaGenics, Inc. No right under any other patent claims to methods, apparatus or reagents is conveyed expressly, by implication, or by estoppel. This product is intended for Research Use Only and may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products without prior written approval of SomaGenics, Inc. For more information about commercial rights, please contact SomaGenics Business Development at bd@somagenics.com.

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Applications: preparation for Next-generation sequencing of small RNAs
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Applications: preparation for Next-generation sequencing of small RNAs
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Applications: preparation for Next-generation sequencing of small RNAs
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