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Active Endosome Escape Based Transfection Reagent
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Cas9 Ribonucleoprotein (RNP) Delivery - Viromer® CRISPR

Cas9 Ribonucleoprotein (RNP) Delivery - Viromer® CRISPR

Cambridge Bioscience offers a highly effective and efficient method for in vitro delivery of Cas9 ribonucleoprotein (RNP) for genome editing. Offering a powerful alternative for DNA-based approaches for challenging cells, the versatile Viromer® CRISPR reagent is fully compatible with suspension cells and optimised for the in vitro delivery of Cas9 RNP ( a pre-complex of the Cas9 endonuclease with one specific guide RNA).

Benefits Of Using Viromer® CRISPR
• High transfection efficiency mediated by polymer nanoparticles
• Direct delivery of Cas proteins complexed with guide RNAs
• Increased efficiency of gene editing compared to lipofection or electroporation
• Reduction in off-target effects relative to nuclease kinetics
• Low impact on cell viability & physiology
• Simple protocol
• Compatible with serum & antibiotics

Delivery of pre-formed RNP complex is safer & faster than plasmid-based transfection

Viromer RNP delivery

CRISPR components are directly active upon transfection, bypassing the need to use the cell machinery and skipping the assemblage step in the cytosol. Transient action limits off-target cleavage, with no integration of the Cas9 gene into the cell genome, allowing for better control of the Cas9 activity.
 

VIROMER® CRISPR for RNP delivery

CRISPR/Cas9 mediated knock-out of RFP expression in stable HEK293T. Up to 60% reduction of RFP expression was observed 7days post-transfection of RNP complex of Cas9 and gRNA (source: OriGene, US).

Applications
• In vitro delivery of Cas9
• Cancer research
• Stem cell research
• Cell signalling

Which Viromer® for CRISPR/Cas9 experiments?
If you are transfecting plasmid DNA or mRNA encoding Cas9 protein, we recommend using the Viromer® RED. To find out more, please click Here.

For transfection of guide RNA into Cas9-stable cells, please try Viromer® BLUE or Viromer® GREEN. To find out more, please click Here.

The success of RNP delivery and subsequent genome editing effect will depend on transfection efficiency. It will vary with cell types and can require optimisation. To check Viromer® transfection efficiency by cell type, please contact our Lipocalyx specialist.

Free Sample
To request a free sample, please click Here.

Material Available For Download
Viromer® CRISPR Brochure
Viromer® CRISPR Protocol
A549: Transfection of Cas9/gRNA RNP
C2C12: Transfection of Cas9/gRNA RNP
HUVEC: Transfection of Cas9/gRNA RNP
PDAC: Transfection of Cas9/gRNA RNP

Products

Note: availability depends on country - see product detail page.

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Applications: transfection, RNP delivery
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Applications: transfection, RNP delivery
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Applications: transfection, RNP delivery
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Applications: transfection, RNP delivery
Doaa Lipocalyx
Lipocalyx - Novel Active Endosome Escape Based Transfection Reagents