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Small RNA Library Preparation For NGS

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Custom Small RNA Library Preparation & Sequencing Services

Custom Small RNA Library Preparation & Sequencing Services

Cambridge Bioscience offers small RNA sequencing and library preparation services from SomaGenics. Based on RealSeq®-AC technology, all sequencing prices include a minimum of 5 million reads with a read length of up to 75 bp. For longer read length, or for analysis of sequencing results by SomaGenics, please contact our SomaGenics specialist.

To find out more about the RealSeq®-AC technology, please Click Here.

Benefits Of Using These sRNA Library Prep & Sequencing Services
• Based on the RealSeq®-AC technology
• Eliminates bias-induced over/under represented small RNAs
• Efficient & gel-free workflow
• Optimised for inputs between 1 ng - 100 ng total RNA
• Detects more miRNAs in total RNA than other miRNA library preparation kits
• Lower sequencing costs due to the reduced number of irrelevant sequencing reads

Services Available
1. Small RNA Sequencing From Total RNA

RNA Sample Type

Service Includes

Sample Size Required

Tissues or cell

Library prep using RealSeq®-ACplus technology, library QC & sequencing on an Illumina platform (MiniSeq or Next Seq). Please enquire if you would like SomaGenics to perform RNA preparation. 5 ng/library at a concentration of 1 ng/ul

From biofluids

Library prep using RealSeq®-biofluids technology, library QC & sequencing on an Illumina platform (MiniSeq or Next Seq) RNA should ideally be isolated from a sample size of 200 ul or more (minimum input of 50 ul)

Direct from biofluid sample

RNA extraction from biofluids, library prep using RealSeq®-biofluids technology, library QC & sequencing on an Illumina platform (MiniSeq or Next Seq) Optimal sample size to be provided is 200 ul (minimum sample size 50 ul). For biofluids other than plasma or serum, please discuss with our SomaGenics specialist.

Sample quality is essential for successful sequencing results. Most RNA prepared from biofluids is difficult to quantify and QC. SomaGenics will prepare the sequencing library and perform sequencing only if the QC of the library is satisfactory.

NB: Heparin inhibits enzymes in the library preparation steps so samples preserved in heparin will not give satisfactory results

Total RNA Source

Number of samples

Catalogue number

From tissues or cells Small RNA sequencing for 1 to 10 samples 500-totalRNA10
  Small RNA sequencing for 11 to 20 samples 500-totalRNA20
  Small RNA sequencing for over 20 samples 500-totalRNA20plus
From biofluids Small RNA sequencing for 1 to 10 samples 600-totalRNA10
  Small RNA sequencing for 11 to 19 samples 600-totalRNA20
  Small RNA sequencing for over 20 samples 600-totalRNA20plus
Direct from biofluid Small RNA sequencing for 1 to 10 samples 600-BF10
  Small RNA sequencing for 10 to 20 samples 600-BF20
  Small RNA sequencing for over 20 samples 600-BF20plus

2. Library Preparation
• Tissue or cells - library preparation using RealSeq®-ACplus technology & library QC
• Biofluids - library preparation using RealSeq®-biofluids technology & library QC
• Sample quality is essential for successful library preparation
• Ideal submitted sample size is 200 ul or more (minimum 50 ul)

For further details, please contact our SomaGenics specialist.

Library prep source

Number of samples

Catalogue number

Tissues or cells Library preparation for 1 to 10 samples 500-libraryPrep10
  Library preparation for 11 to 20 samples 500-libraryPrep20
  Library preparation for over 20 samples 500-libraryPrep20plus
Biofluids Library preparation for 1 to 10 samples 600-libraryPrep10
  Library preparation for 11 to 20 samples 600-libraryPrep20)
  Library preparation for over 20 samples 600-libraryPrep20plus

3. Bioinformatic Analyses
Bioinformatic analysis of SomaGenics sequencing results is available. For further details, please contact our SomaGenics specialist.

Request A Quotation
Please complete the enquiry form Here

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