TransIT-VirusGEN®: recombinant AAV & lentiviral production

TransIT-VirusGEN® is a high-performance transfection reagent for the reliable and efficient delivery of packaging and transfer vectors to adherent or suspension HEK293 cell types. With a streamlined recombinant adeno-associated virus (AAV) and lentivirus production workflow and highly efficient DNA delivery, TransIT-VirusGEN® can be used with a variety of cell culture systems.

Benefits of using TransIT-VirusGEN
• Consistent high virus titre production
• Compatible with adherent or suspension HEK293 cells
• Can be scaled efficiently
• Streamlined virus generation workflow
• Compatible with different virus & cell culture systems

Read more about the preclinical grade alternative, TransIT-VirusGEN® SELECT, which is ideal large-scale production of viral vectors for clinical trials.

TransIT-VirusGEN outperforms competitor reagents in suspension & adherent AAV cell cultures

Suspension (A) or adherent cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio) with the following reagents: TransIT-VirusGEN (2:1, vol:wt), Lipofectamine® 2000 (3:1), Lipofectamine® 3000 (3:3:1) or 25 kDa PEI (4:1). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT flow cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT-VirusGEN® enables broad scalability (AAV)

Suspension (A) or adherent cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio) using the TransIT-VirusGEN transfection reagent (2:1, vol:wt) at the indicated volumes per culture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. Error bars represent the standard deviation of triplicate wells, flasks, or dishes.

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Material available for download
TransIT-VirusGEN® transfection reagent protocol
TransIT-VirusGEN® tansfection reagent quick reference protocol for AAV generation
TransIT-VirusGEN® transfection reagent SDS

Lipofectamine is a registered trademark of Life Technologies Corporation
Guava® and EasyCyte™are registered trademarks of Guava Technologies, Inc.