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Requerst A Free TransIT-VirusGEN® Sample

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Recombinant AAV & Lentiviral Production - TransIT-VirusGEN®

Recombinant AAV & Lentiviral Production - TransIT-VirusGEN®

Cambridge Bioscience offers a transfection reagent for reliable and efficient delivery of packaging and transfer vectors to adherent or suspension HEK293 cell types. With a streamlined recombinant adeno-associated virus (AAV) and lentivirus production workflow and highly efficient DNA delivery, TransIT-VirusGEN® can be used with a variety of cell culture systems.

Benefits Of Using TransIT-VirusGEN®
• Consistent high virus titre production
• Compatible with adherent or suspension HEK293 cells
• Can be scaled efficiently
• Streamlined virus generation workflow
• Compatible with different virus & cell culture systems

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures

AAV Production in Suspension 293-F Cell Culture

AAV Production in Adherent 293T/17 Cell Culture

Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 µg/ml, Agilent Technologies) with the following reagents: TransIT-VirusGEN® (2:1, vol:wt), Lipofectamine® 2000 (3:1, Thermo Fisher), Lipofectamine® 3000 (3:3:1, Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT-VirusGEN® Enables Broad Scalability (AAV)

TransIT-VirusGEN® Enables Broad Scalability (AAV)

Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 total µg/ml, Agilent Technologies) using the TransIT-VirusGEN® Transfection Reagent (2:1 reagent-to-DNA ratio (vol:wt), Mirus) at the indicated volumes per culture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells.  Error bars represent the standard deviation of triplicate wells, flasks, or dishes.

Free Sample
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Material Available For Download
TransIT-VirusGEN® Transfection Reagent Protocol
TransIT-VirusGEN® Transfection Reagent Quick Reference Protocol for AAV Generation
TransIT-VirusGEN® Transfection Reagent SDS

Lipofectamine is a registered trademark of Life Technologies Corporation

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