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DNA Methylation Library Prep Kit

All-in-One Stabilisation DNA / RNA Shield™ Reagent
Quick, High Quality Total DNA Purification Kits

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Urine Conditioning Buffer™

Urine Conditioning Buffer™

This solution from Zymo Research stabilises nucleic acids in urine for up to a month during ambient temperature storage. With the possibility to be added to any collection device, the Urine Conditioning Buffer™ (UCB™) preserves DNA and RNA whilst being compatible with sensitive applications including next-generation sequencing.

Benefits of using the Urine Conditioning Buffer
• Stabilises and preserves DNA and RNA in urine
• Stabilises at ambient temperatures for up to one month
• No need for refrigeration or specialised equipment
• Compatible with any collection device
• Facilitates pelleting of both cellular and cell-free nucleic acids from large volume urine samples
• Inhibits microbial growth during long-term (cold-free) storage of urine samples

Urine Conditioning Buffer provides superior preservation vs. conventional methods

Urine Conditioning Buffer™ Provides Superior Preservation vs. Conventional Methods

Urine (with or without UCB) was preserved using different storage conditions: room temperature (RT), -20⁰C and -80⁰C. HeLa cells were spiked into urine before starting the RNA experiment. After two weeks of storage, total DNA (yellow) and total RNA (green) were purified. Corresponding fold change of preserved nucleic acids was obtained from qPCR analysis. Experiment was performed in technical duplicates.

Urine Conditioning Buffer prevents microbial growth at ambient temperature

Urine Conditioning Buffer™ Prevents Microbial Growth At Ambient Temperature

Urine was stored with and without UCB for six days. On day six, microbial colony formation was determined by plating 500 µl urine on LB agar, followed by incubation at 37⁰C overnight.

ApplicationsUCB
• Next-generation sequencing
• qPCR
• Microarray

Material available for download
Urine Conditioning Buffer™ protocol
Urine Conditioning Buffer™ SDS

Products

Note: availability depends on country - see product detail page.

Speak To Our Zymo Research Specialist Emma