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Lentiviral Plasmid & Pre-Packaged Virus Reagents – Lenti-Labeler™

Lentiviral Plasmid & Pre-Packaged Virus Reagents – Lenti-Labeler™

Cambridge Bioscience offers an easy way to label cells in vitro for downstream in vivo applications using Lenti-Labeler™ vectors, which come with a range of selection markers (Puro and Blasticidin), reporter genes (GFP, RFP, BFP and luciferase) and two different promoters (CMV and EF1a).  With their sequences verified, all of the Lenti-Labeler™ vectors are based on time-tested and proven 3rd generation backbones to deliver high quality and performance for nearly all potential cell labelling applications.

Benefits Of Using The Lenti-Labeler™
• 3rd generation lentivector technology for optimal virus titres
• Sequence-verified from LTR to LTR and expression-verified
• Flexible

Lenti-Labeler construct

The pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct expresses copepod GFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagable, sequence-verified plasmid DNA (Cat.# LL100A-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL100VA-1), the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.

Available Lenti-Labeler™ Constructs

Cat number Construct Promoter Reporter Selection
LL100PA-1/LL100VA-1 pLL-CMV-GFP-T2A-Puro CMV GFP Puromycin
LL105PA-1/LL105VA-1 pLL-CMV-GFP-T2A-Blast CMV GFP Blasticidin
LL110PA-1/LL110VA-1 pLL-CMV-RFP-T2A-Puro CMV RFP Puromycin
LL115PA-1/LL115VA-1 pLL-CMV-RFP-T2A-Blast CMV RFP Blasticidin
LL120PA-1/LL120VA-1 pLL-CMV-BFP-T2A-Puro CMV BFP Puromycin
LL125PA-1/LL125VA-1 pLL-CMV-BFP-T2A-Blast CMV BFP Blasticidin
LL150PA-1/LL150VA-1 pLL-CMV-Luciferase-T2A-Puro CMV Luciferase Puromycin
LL200PA-1/LL200VA-1 pLL-EF1α-GFP-T2A-Puro EF1α GFP Puromycin
LL205PA-1/LL205VA-1 pLL-EF1α-GFP-T2A-Blast EF1α GFP Blasticidin
LL210PA-1/LL210VA-1 pLL-EF1α-RFP-T2A-Puro EF1α RFP Puromycin
LL215PA-1/LL215VA-1 pLL-EF1α-RFP-T2A-Blast EF1α RFP Blasticidin
LL220PA-1/LL220VA-1 pLL-EF1α-BFP-T2A-Puro EF1α BFP Puromycin
LL225PA-1/LL225VA-1 pLL-EF1α-BFP-T2A-Blast EF1α BFP Blasticidin
LL250PA-1/LL250VA-1 pLL-EF1α-Luciferase-T2A-Puro EF1α Luciferase Puromycin

Lenti-Labeler™ constructs reliably and efficiently label cells

Lenti-Labeler™ constructs reliably and efficiently label cells

Comparison of the number of fluorescently-labelled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labelling efficiency of the Lenti-Labeler™ constructs.

While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct is compatible with most second and third generation packaging plasmid mixes, we recommend the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titres and infection of target cells.

Material Available For Download
Lenti-Labeler™ User Manual

Products

Note: availability depends on country - see product detail page.

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Tonbo Biosciences